LOCUS       sequence-131616-         429 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mouse targeting vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Robles-Oteiza C, Taylor S, Yates T, Cicchini M, Lauderback B, 
            Cashman CR, Burds AA, Winslow MM, Jacks T, Feldser DM
  TITLE     Recombinase-based conditional and reversible gene regulation via XTR
            alleles.
  JOURNAL   Nat Commun. 2015 Nov 5;6:8783. doi: 10.1038/ncomms9783.
  PUBMED    26537451
REFERENCE   2  (bases 1 to 429)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-11-5"; volume: "6:8783. doi"; pages: " 
            10.1038/ncomms9783"
COMMENT     Sequence Label: pNeoXTR f2
FEATURES             Location/Qualifiers
     source          1..429
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    357..390
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    357..390
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct
       61 gaagcactgc acnccgtagg tcagggtggt cacnagggtg ggccagggca cgggcagctt
      121 gccggtggtg cagatgaact tcagggtcag cttgccgtat gtggcatctc cctcgccctc
      181 gccggacacn ctgaacttgt ggccgtttac atcgccgtcc agctcgacca ggatgggcac
      241 caccccggtg aacagctcct cgcccttgct caccatcctg gaaaaaagaa atgcgaagat
      301 tgcgcctgcg catcaataaa ataaaagatt ttatttagtc tccagaaaaa gcccggataa
      361 cttcgtataa ggtatcctat acgaagttat ccattcaggc tgtgctagca tcaatggcat
      421 ggcacaaag
//
