LOCUS       sequence-131619-       10675 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Generating transgenic flies for 4-hydroxytamoxifen-inducible DamID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10675)
  AUTHORS   Pindyurin AV, Pagie L, Kozhevnikova EN, van Arensbergen J, van 
            Steensel B
  TITLE     Inducible DamID systems for genomic mapping of chromatin proteins in
            Drosophila.
  JOURNAL   Nucleic Acids Res. 2016 Jul 8;44(12):5646-57. doi: 
            10.1093/nar/gkw176. Epub 2016 Mar 21.
  PUBMED    27001518
REFERENCE   2  (bases 1 to 10675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkw176"; journalName: "Nucleic Acids Res"; date: 
            "2016-07-8- 8"; volume: "44"; issue: "12"; pages: "5646-57"
COMMENT     Sequence Label: p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]Myc[clo...
FEATURES             Location/Qualifiers
     source          1..10675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    7..40
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        42..494
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     441..462
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             2593..2622
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     intron          2747..2812
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2942..2962
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3234..3368
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3271..3290)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3325..3344
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    3397..3466
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(3685..3703)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3724..3740)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3724..3740)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3737..3759)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3748..3764
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3772..3778,3779..3796,3797..3802))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3817..3838
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3955..3972)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4126..4714)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4206..4225)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4885..5676,5677..5745))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5508..5527
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5746..5850)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5877..6332
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5964..5983)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6174..6195
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6458..6480
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6472..6489
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6473..6489
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6496..6515
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6496..6514
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            6539..10675
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(6548..6566)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(7018..7089,7491..7764,7839..8493,8555..8870,9091..9222,
                     9293..9907)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 gctagcataa cttcgtataa tgtatgctat acgaagttat cctagaatcc caaaacaaac
       61 tggttattgt ggtaggtcat ttgtttggca gaaagaaaac tcgagaaatt tctctggccg
      121 ttattcgtta ttctctcttt tctttttggg tctctccctc tctgcactaa tgctctctca
      181 ctctgtcaca cagtaaacgg catactgctc tcgttggttc gagagagcgc gcctcgaatg
      241 ttcgcgaaaa gagcgccgga gtataaatag aggcgcttcg tctacggagc gacaattcaa
      301 ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa caagcgcagc
      361 tgaacaagct aaacaatctg cagtaaagtg caagttaaag tgaatcaatt aaaagtaacc
      421 agcaaccaag taaatcaact gcaactactg aaatctgcca agaagtaatt attgaataca
      481 agaagagaac tctgaatagg gaattgggaa ttcaacatga agaaaaatcg cgcttttttg
      541 aagtgggcag ggggcaagta tcccctgctt gatgatatta aacggcattt gcccaagggc
      601 gaatgtctgg ttgagccttt tgtaggtgcc gggtcggtgt ttctcaacac cgacttttct
      661 cgttatatcc ttgccgatat caatagcgac ctgatcagtc tctataacat tgtgaagatg
      721 cgtactgatg agtacgtaca ggccgcacgc gagctgtttg ttcccgaaac aaattgcgcc
      781 gaggtttact atcagttccg cgaagagttc aacaaaagcc aggatccgtt ccgtcgggcg
      841 gtactgtttt tatatttgaa ccgctacggt tacaacggcc tgtgtcgtta caattgcctt
      901 gccgagggta cccgaatctt cgatccggtc actggtacaa cgcatcgcat cgaggatgtt
      961 gtcgatgggc gcaagcctat tcatgtcgtg gctgttgcca aggacggaac gctgctcgcg
     1021 cggcccgtgg tgtcctggtt cgaccaggga acgcgggatg tgatcgggtt gcggatcgcc
     1081 ggtggcgcca tcgtgtgggc gacacccgat cacaaggtgc tgacagagta cggctggcgt
     1141 gccgccgggg aactccgcaa gggagacagg gtggccggac cgggtggttc tggtaacagc
     1201 ctggccttgt ccctgacggc cgaccagatg gtcagtgcct tgttggatgc tgagcccccc
     1261 atactctatt ccgagtatga tcctaccagt cccttcagtg aagcttcgat gatgggctta
     1321 ctgaccaacc tggcagacag ggagctggtt cacatgatca actgggcgaa gagggtgcca
     1381 ggctttgtgg atttgaccct ccatgatcag gcccaccttc tagaatgtgc ctggctagag
     1441 atcctgatga ttggtctcgt ctggcgctcc atggagcacc cagggaagct actgtttgct
     1501 cctaacttgc tcttggacag gaaccaggga aaatgtgtag agggcatggt ggagatcttc
     1561 gacatgctgc tggctacatc atctcggttc cgcatgatga atctgcaggg agaggagttt
     1621 gtgtgcctca aatctattat tttgcttaat tctggagtgt acacatttct gtccagcacc
     1681 ctgaagtctc tggaagagaa ggaccatatc caccgagccc tggacaagat cacggacact
     1741 ttgatccacc tgatggccaa ggcaggcctg accctgcagc agcagcacca gcggctggcc
     1801 cagctcctcc tcatcctctc ccacatcagg cacatgagta acaaaggaat ggagcatctg
     1861 tacagcatga agtacacgaa cgtggtgccc ctctatgacc tgctgctgga gatgctggac
     1921 gcccaccgcc tacatgcggg tggttctggt gctagccgcg tgcaggcgtt cgcggatgcc
     1981 ctggatgaca aattcctgca cgacatgctg gcggaagaac tccgctattc cgtgatccga
     2041 gaagtgctgc caacgcggcg ggcacgaacg ttcgacctcg aggtcgagga actgcacacc
     2101 ctcgtcgccg aaggggttgt cgtgcacaac tgtcgcggtg agtttaacgt gccgttcggc
     2161 cgctacaaaa aaccctattt cccggaagca gagttgtatc acttcgctga aaaagcgcag
     2221 aatgcctttt tctattgtga gtcttacgcc gatagcatgg cgcgcgcaga tgatgcatcc
     2281 gtcgtctatt gcgatccgcc ttatgcaccg ctgtctgcga ccgccaactt tacggcgtat
     2341 cacacaaaca gttttacgct tgaacaacaa gcgcatctgg cggagatcgc cgaaggtctg
     2401 gttgagcgcc atattccagt gctgatctcc aatcacgata cgatgttaac gcgtgagtgg
     2461 tatcagcgcg caaaattgca tgtcgtcaaa gttcgacgca gtataagcag caacggcggc
     2521 acacgtaaaa aggtggacga actgctggct ttgtacaaac caggagtcgt ttcacccgcg
     2581 aaaaaagccg gtgaacagaa actcatctct gaagaggatc tgtaaggcgc gccatttaaa
     2641 tggccggccg cgatcgcggc cgctctagag gatctttgtg aaggaacctt acttctgtgg
     2701 tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt
     2761 tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac
     2821 ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg
     2881 ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc
     2941 tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt
     3001 tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa
     3061 ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tatttgatgt atagtgcctt
     3121 gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     3181 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     3241 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     3301 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     3361 tctggatcca ctagtgtcga cgatgtaggt cacggtctcg aagccgcggt gcgggtgcca
     3421 gggcgtgccc ttgggctccc cgggcgcgta ctccacctca cccatctggt ccatcatgat
     3481 gaacgggtcg aggtggcggt agttgatccc ggcgaacgcg cggcgcaccg ggaagccctc
     3541 gccctcgaaa ccgctgggcg cggtggtcac ggtgagcacg ggacgtgcga cggcgtcggc
     3601 gggtgcggat acgcggggca gcgtcagcgg gttctcgacg gtcacggcgg gcatgtcgac
     3661 actagttcta gccagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc
     3721 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     3781 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     3841 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     3901 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     3961 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     4021 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     4081 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     4141 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     4201 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     4261 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     4321 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     4381 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     4441 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     4501 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     4561 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     4621 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     4681 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     4741 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     4801 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     4861 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     4921 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     4981 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     5041 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     5101 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     5161 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     5221 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     5281 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     5341 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     5401 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     5461 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     5521 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     5581 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     5641 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     5701 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     5761 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     5821 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc
     5881 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     5941 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     6001 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     6061 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     6121 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     6181 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     6241 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     6301 gaattttaac aaaatattaa cgcttacaat ttccattcgc cattcaggct gcgcaactgt
     6361 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     6421 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     6481 acggccagtg aattgtaata cgactcacta tagggcgaat tgggtacgta ccgggcccct
     6541 agtatgtatg taagttaata aaacccattt ttgcggaaag tagataaaaa aaacattttt
     6601 tttttttact gcactggata tcattgaact tatctgatca gttttaaatt tacttcgatc
     6661 caagggtatt tgatgtacca ggttctttcg attacctctc actcaaaatg acattccact
     6721 caaagtcagc gctgtttgcc tccttctctg tccacagaaa tatcgccgtc tctttcgccg
     6781 ctgcgtccgc tatctctttc gccaccgttt gtagcgttac gtagcgtcaa tgtccgcctt
     6841 cagttgcatt ttgtcagcgg tttcgtgacg aagctccaag cggtttacgc catcaattaa
     6901 acacaaagtg ctgtgccaaa actcctctcg cttcttattt ttgtttgttt tttgagtgat
     6961 tggggtggtg attggttttg ggtgggtaag caggggaaag tgtgaaaaat cccggcaatg
     7021 ggccaagagg atcaggagct attaattcgc ggaggcagca aacacccatc tgccgagcat
     7081 ctgaacaatg tgagtagtac atgtgcatac atcttaagtt cacttgatct ataggaactg
     7141 cgattgcaac atcaaattgt ctgcggcgtg agaactgcga cccacaaaaa tcccaaaccg
     7201 caattgcaca aacaaatagt gacacgaaac agattattct ggtagctgtt ctcgctatat
     7261 aagacaattt ttgagatcat atcatgatca agacatctaa aggcattcat tttcgactat
     7321 attctttttt acaaaaaata taacaaccag atattttaag ctgatcctag atgcacaaaa
     7381 aataaataaa agtataaacc tacttcgtag gatacttcgg ggtacttttt gttcggggtt
     7441 agatgagcat aacgcttgta gttgatattt gagatcccct atcattgcag ggtgacagcg
     7501 gagcggcttc gcagagctgc attaaccagg gcttcgggca ggccaaaaac tacggcacgc
     7561 tccggccacc cagtccgccg gaggactccg gttcagggag cggccaacta gccgagaacc
     7621 tcacctatgc ctggcacaat atggacatct ttggggcggt caatcagccg ggctccggat
     7681 ggcggcagct ggtcaaccgg acacgcggac tattctgcaa cgagcgacac ataccggcgc
     7741 ccaggaaaca tttgctcaag aacggtgagt ttctattcgc agtcggctga tctgtgtgaa
     7801 atcttaataa agggtccaat taccaatttg aaactcagtt tgcggcgtgg cctatccggg
     7861 cgaacttttg gccgtgatgg gcagttccgg tgccggaaag acgaccctgc tgaatgccct
     7921 tgcctttcga tcgccgcagg gcatccaagt atcgccatcc gggatgcgac tgctcaatgg
     7981 ccaacctgtg gacgccaagg agatgcaggc caggtgcgcc tatgtccagc aggatgacct
     8041 ctttatcggc tccctaacgg ccagggaaca cctgattttc caggccatgg tgcggatgcc
     8101 acgacatctg acctatcggc agcgagtggc ccgcgtggat caggtgatcc aggagctttc
     8161 gctcagcaaa tgtcagcaca cgatcatcgg tgtgcccggc agggtgaaag gtctgtccgg
     8221 cggagaaagg aagcgtctgg cattcgcctc cgaggcacta accgatccgc cgcttctgat
     8281 ctgcgatgag cccacctccg gactggactc atttaccgcc cacagcgtcg tccaggtgct
     8341 gaagaagctg tcgcagaagg gcaagaccgt catcctgacc attcatcagc cgtcttccga
     8401 gctgtttgag ctctttgaca agatccttct gatggccgag ggcagggtag ctttcttggg
     8461 cactcccagc gaagccgtcg acttcttttc ctagtgagtt cgatgtgttt attaagggta
     8521 tctagcatta cattacatct caactcctat ccagcgtggg tgcccagtgt cctaccaact
     8581 acaatccggc ggacttttac gtacaggtgt tggccgttgt gcccggacgg gagatcgagt
     8641 cccgtgatcg gatcgccaag atatgcgaca attttgctat tagcaaagta gcccgggata
     8701 tggagcagtt gttggccacc aaaaatttgg agaagccact ggagcagccg gagaatgggt
     8761 acacctacaa ggccacctgg ttcatgcagt tccgggcggt cctgtggcga tcctggctgt
     8821 cggtgctcaa ggaaccactc ctcgtaaaag tgcgacttat tcagacaacg gtgagtggtt
     8881 ccagtggaaa caaatgatat aacgcttaca attcttggaa acaaattcgc tagattttag
     8941 ttagaattgc ctgattccac acccttctta gtttttttca atgagatgta tagtttatag
     9001 ttttgcagaa aataaataaa tttcatttaa ctcgcgaaca tgttgaagat atgaatatta
     9061 atgagatgcg agtaacattt taatttgcag atggttgcca tcttgattgg cctcatcttt
     9121 ttgggccaac aactcacgca agtgggcgtg atgaatatca acggagccat cttcctcttc
     9181 ctgaccaaca tgacctttca aaacgtcttt gccacgataa atgtaagtct tgtttagaat
     9241 acatttgcat attaataatt tactaacttt ctaatgaatc gattcgattt aggtgttcac
     9301 ctcagagctg ccagttttta tgagggaggc ccgaagtcga ctttatcgct gtgacacata
     9361 ctttctgggc aaaacgattg ccgaattacc gctttttctc acagtgccac tggtcttcac
     9421 ggcgattgcc tatccgatga tcggactgcg ggccggagtg ctgcacttct tcaactgcct
     9481 ggcgctggtc actctggtgg ccaatgtgtc aacgtccttc ggatatctaa tatcctgcgc
     9541 cagctcctcg acctcgatgg cgctgtctgt gggtccgccg gttatcatac cattcctgct
     9601 ctttggcggc ttcttcttga actcgggctc ggtgccagta tacctcaaat ggttgtcgta
     9661 cctctcatgg ttccgttacg ccaacgaggg tctgctgatt aaccaatggg cggacgtgga
     9721 gccgggcgaa attagctgca catcgtcgaa caccacgtgc cccagttcgg gcaaggtcat
     9781 cctggagacg cttaacttct ccgccgccga tctgccgctg gactacgtgg gtctggccat
     9841 tctcatcgtg agcttccggg tgctcgcata tctggctcta agacttcggg cccgacgcaa
     9901 ggagtagccg acatatatcc gaaataactg cttgtttttt ttttttacca ttattaccat
     9961 cgtgtttact gtttattgcc ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa
    10021 acaagatatg acctatagaa tacaagtatt tccccttcga acatccccac aagtagactt
    10081 tggatttgtc ttctaaccaa aagacttaca cacctgcata ccttacatca aaaactcgtt
    10141 tatcgctaca taaaacaccg ggatatattt tttatataca tacttttcaa atcgcgcgcc
    10201 ctcttcataa ttcacctcca ccacaccacg tttcgtagtt gctctttcgc tgtctcccac
    10261 ccgctctccg caacacattc accttttgtt cgacgacctt ggagcgactg tcgttagttc
    10321 cgcgcgattc ggttcgctca aatggttccg agtggttcat ttcgtctcaa tagaaattag
    10381 taataaatat ttgtatgtac aatttatttg ctccaatata tttgtatata tttccctcac
    10441 agctatattt attctaattt aatattatga ctttttaagg taattttttg tgacctgttc
    10501 ggagtgatta gcgttacaat ttgaactgaa agtgacatcc agtgtttgtt ccttgtgtag
    10561 atgcatctca aaaaaatggt gggcataata gtgttgttta tatatatcaa aaataacaac
    10621 tataataata agaatacatt taatttagaa aatgcttgga tttcactgga actag
//
