LOCUS       sequence-13163-e        3582 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1185-HQ858807
FEATURES             Location/Qualifiers
     source          1..3582
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1139..1238)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1255..1274)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1256..1274)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1279..1295)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1279..1295)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1397..1416)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1408..2217
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1475..1494)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2364..2952
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2853..2872
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3106..3123
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3282..3309)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3401..3487)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3536..3558
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3550..3567
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3551..3567
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atggcagatg ctagttcaag gactgacaca tcgattgttg tagacaacga cgacaaaaac
      181 caccagttag aaaacggaca tagtggtgca gtcatggctt ctaactcttc agatagatct
      241 gacagatctg acaaacttat ggaccaaaag acaattcggc ggcttgctca aaatcgtgag
      301 gcagcaagaa aaagtcggct gaggaaaaag gcatatgtgc aacaactaga gagcagtaag
      361 ctgaagcttg cacagctaga gcaggaactt cagaaagctc gtcagcaggg aatcttcatc
      421 tctagctctg gagaccagac ccatgccatg agtggaaatg gggcattgac ttttgactta
      481 gaatacacta gatggctcga ggagcaaaat aagcagataa atgagttgag gacagcagtg
      541 aatgctcatg caagtgacag tgaccttcgt cttattgttg atggcataat ggcgcattat
      601 gacgaggtat tcaaggttaa gggtgtagct gcaaaggccg atgtgtttca tatactttca
      661 ggcatgtgga agacacccgc agaaagatgc ttcctgtggc ttggtggttt ccgtccatct
      721 gagcttctaa agctcctagc aaatcacctc gaacctttaa ccgagcagca gttgctggga
      781 ttaaacaacc tccaggaatc ttctcagcag gcggaggatg cactttcaca aggtatggaa
      841 gcactgcagc aatctctggc agatactttg gctggatctc tcggttcatc agggtcttct
      901 gggaatgtgg cgaactacat gggtcagatg gcaatggcca tgggtaaact aggaacgctc
      961 gagaatttcc tttgccaggc ggacaacctg cgacagcaga cattgcatca aatgcaacga
     1021 attctgacga tccggcaagc ctcgcgtgct cttcttgcca tacacgatta cttttcacgc
     1081 ttgcgtgctt tgagttcgct gtggcttgct aggccacggg agaagggtgg gcgcgccgac
     1141 ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac
     1201 gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat
     1261 agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat
     1321 ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct
     1381 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg
     1441 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
     1501 gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt
     1561 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
     1621 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
     1681 gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat
     1741 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
     1801 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
     1861 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
     1921 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc
     1981 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
     2041 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     2101 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     2161 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca
     2221 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca
     2281 agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
     2341 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     2401 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2461 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     2521 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     2581 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     2641 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     2701 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga
     2761 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2821 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2881 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2941 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3001 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     3061 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     3121 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     3181 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     3241 aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga
     3301 tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc
     3361 cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag
     3421 cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt
     3481 ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag
     3541 tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cc
//
