LOCUS       sequence-13164-e        2985 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1186-HQ858808
FEATURES             Location/Qualifiers
     source          1..2985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(542..641)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(658..677)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(659..677)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(682..698)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(682..698)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(800..819)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             811..1620
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(878..897)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1767..2355
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2256..2275
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2509..2526
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2685..2712)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2804..2890)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2939..2961
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2953..2970
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2954..2970
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atggccggga gagggaaggc gatcggctcg ggcgccgcca agaaggccat gtcgcggagc
      181 tccaaggccg ggctccagtt ccccgtgggc aggatcgccc ggttcttgaa ggccggcaag
      241 tacgccgaga gggtcggcgc cggcgccccg gtctacctcg ccgccgtcct cgagtacctc
      301 gccgctgagg ttcttgagct cgccgggaac gcggcgaggg acaacaagaa gacccgcata
      361 gtgccccgcc acatccagct cgccgtccgc aacgacgagg agctatcccg cctgctcggc
      421 acggtcacaa tcgccagcgg aggcgtcatg cccaacatcc acaacctgct cctccccaag
      481 aaggccggcg gcagcgccaa ggcggcggcc ggcgacgatg acaacaaggg tgggcgcgcc
      541 gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc
      601 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc
      661 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
      721 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
      781 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
      841 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
      901 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
      961 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     1021 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     1081 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa
     1141 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     1201 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     1261 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     1321 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     1381 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     1441 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     1501 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     1561 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     1621 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     1681 gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     1741 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1801 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1861 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     1921 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1981 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2041 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2101 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat
     2161 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2221 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2281 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     2341 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     2401 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     2461 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     2521 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     2581 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     2641 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     2701 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     2761 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     2821 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc
     2881 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc
     2941 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccc
//
