LOCUS       sequence-13165-e        3432 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1187-HQ858809
FEATURES             Location/Qualifiers
     source          1..3432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(989..1088)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1105..1124)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1106..1124)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1129..1145)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1129..1145)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1247..1266)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1258..2067
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1325..1344)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2214..2802
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2703..2722
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2956..2973
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3132..3159)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3251..3337)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3386..3408
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3400..3417
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3401..3417
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atggaagcag acgcgagcca tacacccacc acctcctcct ccgtctccgt ctccttctcc
      181 tcctcgtcgc tgtccacttc ctcctccacc tcctccctcg tcgacaatgg cgcgcaagac
      241 cggcccaaga gctccaagcc caaacacgcc gccaagaagc gcaagagagc ggccgcggag
      301 gaacctgcca atgccgccca cggcgcaggg gaggatacca gcagctgcag caccgacgac
      361 aacgcggcgg cgagcggcaa ggcgcaggcg ggcggcggcg gcggcggcgt cgacagcagc
      421 agcacctgca ccgccgcctc ggcgccgagg agcggcttca agcacccgtc gtaccgcggc
      481 gtgcgccgcc ggagctgggg gaagtgggtg tcggagatcc gggagccccg caagaagtcg
      541 cgcatctggc tgggtacctt ccccaccgcg gagatggcgg cgcgcgccca cgacgtggcc
      601 gcgctcgcca tcaagggccg gaacgcgcac ctcaacttcc cggacagcgc ccacgagctg
      661 ccccgcccgg agtccacctc cccggcagac atccaggccg ccgccgccaa ggctgccgcc
      721 gaggtgcggt gcgaggagga gtcgtcgccg tcgtcgtcgc ccaccgccga gcaacccgag
      781 gaggaagccg cctgccctga cacggtgcac gccgacggcg gccaggacaa tgctctcttc
      841 gacctacccg accttcttct cgacctacgg gacgggctct ggtggtcgcc ggtgtggccg
      901 gcggcactgg cggccgagga gtacgacggc ggcgacgccg tcgtgctcaa tgagccactc
      961 ttgtgggccg agaagggtgg gcgcgccgac ccagctttct tgtacaaagt tggcattata
     1021 agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
     1081 attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt
     1141 tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
     1201 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg
     1261 agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta
     1321 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg
     1381 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
     1441 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc
     1501 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga
     1561 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
     1621 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc
     1681 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg
     1741 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat
     1801 aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac
     1861 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca
     1921 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
     1981 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
     2041 catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag
     2101 agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt
     2161 tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2221 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2281 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2341 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     2401 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2461 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2521 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2581 gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag
     2641 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2701 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2761 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     2821 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     2881 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     2941 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3001 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3061 tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt
     3121 gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag
     3181 tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg
     3241 ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
     3301 aggcccagtc ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc
     3361 gcgttaacgc tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt
     3421 aagctcgggc cc
//
