LOCUS       sequence-13167-e        3390 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1191-HQ858811
FEATURES             Location/Qualifiers
     source          1..3390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(947..1046)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1063..1082)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1064..1082)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1087..1103)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1087..1103)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1205..1224)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1216..2025
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1283..1302)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2172..2760
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2661..2680
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2914..2931
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3090..3117)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3209..3295)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3344..3366
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3358..3375
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3359..3375
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atgcacgcca tcatggcgag gcgatgctct ggtgactact cgactgcagg ccaacgagcc
      181 ggcgaggagg gcggcggcgg cggcggcgcc gggctacggc tgttcggggt gcagctccat
      241 gctgcggcgg ccagctcgcc ggcgtcctac ttgcacaaga gttacagcat ggattgcctg
      301 cggctgcagg tttcttctcc ttcctccttg cagtcgtcgt cgtcgtcgcc gtcgccgttg
      361 acgtcctcgt tgttgctgtc catcgacgag ggctgcgaga ggccagccgc cgacggctac
      421 ctctccgacg ggcctcacgg cgcggcggca accatgcggg agaggaagaa aggagttcca
      481 tggagcgagc aagagcacag gctgttcctg gcggggctgg agaagctggg caagggcgac
      541 tggcgaggca tctcccggag cttcgtcacc accaggacgc ccacccaggt cgccagccat
      601 gcccagaagt tcttcctccg ccacaacagc gccgccaaga agaccaacaa caagcgccgc
      661 tccagcctct tcgacatggt tcaggattgt gacagtggag gaagatctct cgcctcatcc
      721 gatcctgcca ctcgctgcaa caacaacatc tctgcttctc tgtctctcca agtttcgcac
      781 cacaaatcag gtgacagtgc gtggccttcg tcagaaacac catcagtttc agaagcacaa
      841 caaggtcatg gatacggcac tagtcaccat tgctctccgc tggacctgga gctgggcatg
      901 tccctgtcca ccacgccatc catcggaaca aagggtgggc gcgccgaccc agctttcttg
      961 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
     1021 atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat
     1081 tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac
     1141 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt
     1201 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc
     1261 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     1321 gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     1381 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
     1441 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
     1501 accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt
     1561 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     1621 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
     1681 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     1741 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt
     1801 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     1861 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     1921 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     1981 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat
     2041 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca
     2101 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     2161 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2221 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2281 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     2341 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2401 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2461 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2521 gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg
     2581 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2641 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2701 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     2761 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     2821 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     2881 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     2941 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     3001 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
     3061 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
     3121 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
     3181 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
     3241 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
     3301 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt
     3361 aaaacgacgg ccagtcttaa gctcgggccc
//
