LOCUS       sequence-13170-e        3831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1202-HQ858814
FEATURES             Location/Qualifiers
     source          1..3831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1388..1487)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1504..1523)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1505..1523)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1528..1544)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1528..1544)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1646..1665)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1657..2466
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1724..1743)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2613..3201
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3102..3121
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3355..3372
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3531..3558)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3650..3736)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3785..3807
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3799..3816
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3800..3816
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atgcaaactg cagatcattc tgttcctata ggagtgagat tgctcaaatt gcagttatca
      181 accattgagg cttctattcc accagaagca attcaaccat tttggactga tggttatcga
      241 aaatcttggg gtgtgaagtt gcactctaca acttcgttgg aggaaatatt tcagatgttg
      301 acactgctgg aagccgcaat aaaacgggac cacctatctt cagaatttga aacaacatct
      361 gagttgctta acttgaacac acaagataat ccatcccaga atcatgttgg attgtctgga
      421 tctgctgctg tacttccatg ggttcctgac actactgctg cgattgcact acgaatgtta
      481 gacctagact cagcagtatc atacatgcaa aatcaaaaga tggagagaaa tggtggagat
      541 ttcatgaagc ctccatcaag gtttgtggct gttaagaatg cacaagaatt agatccactg
      601 gaaacaaccg gtttagacct ttttgatgga aggtgggcta ctggtagtgg ccgtaggggt
      661 cgtggacgag gaagtagagg gggtagcagg ggaggcagag gccgaagtcg aggtggaagg
      721 gtcccaagag gtattagcat ctcatctagg attggattta aggatgaaaa tgaagcctca
      781 aggaagaaca ctcgtcgagg gcgtactcgt ggccgtggtc gtgggcgagg acgtcgaact
      841 gttaggtcac ggcaaccatc tgagggcaaa ggcagatcaa ttcccaagga aaatttgttg
      901 gggagcttta gcatgcttag caatgcaaag gctgccactg ttgaggagtc tccaaggagc
      961 tcaggtgctg atgaatgggg cttggagaac agaaggccat atattgatgg tgatgagaac
     1021 agttctggat ctcagttaga tcaatcagaa gataatgagg aaaatggcca acccatggat
     1081 gaggaatatg atgagcaggt tccagattat tctagaggct attctggtgg ctccaggccc
     1141 catggcatga tagatgatga tgtgagcgaa gaagaggatg aagatgtgga gggcgatgat
     1201 gatggggagg aagatgatgc ggaccgtgca gttgatgatg ttgatgctga gatggacgag
     1261 gatgatgata taggtgatga tggtgaggat ggtggtgacg gtggtgacgg tgtggaggcg
     1321 aatgctgatg aagatgaagg cggttcctcg tattcatcgg agtacagcga caagggtggg
     1381 cgcgccgacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     1441 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
     1501 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     1561 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
     1621 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     1681 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     1741 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
     1801 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     1861 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     1921 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta
     1981 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     2041 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     2101 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     2161 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     2221 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     2281 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     2341 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     2401 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     2461 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     2521 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg
     2581 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2641 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2701 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     2761 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     2821 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     2881 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     2941 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3001 gagcattgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3061 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3121 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3181 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3241 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3301 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3361 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     3421 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     3481 aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat
     3541 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc
     3601 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac
     3661 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag
     3721 cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg
     3781 ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc c
//
