LOCUS       sequence-131701-         510 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Tang ED, Nunez G, Barr FG, Guan KL
  TITLE     Negative regulation of the forkhead transcription factor FKHR by 
            Akt.
  JOURNAL   J Biol Chem. 1999 Jun 11. 274(24):16741-6.
  PUBMED    10358014
REFERENCE   2  (bases 1 to 510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1999 Jun 11. 274(24):16741-6."
COMMENT     Sequence Label: pcDNA3 Flag FKHR
FEATURES             Location/Qualifiers
     source          1..510
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             99..122
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttaccatgga ctacaaggac gacgatgaca
      121 agggatccga gctcggatct gatgtcacca tggccgaggc gcctcaggtg gtggagatcg
      181 acccggactt cgagccgctg ccccggccgc gctcgtgcac ctggccgctg cccaggccgg
      241 agtttagcca gtccaactcg gccacctcca gcccggcgcc gtcgggcagc gcggctgcca
      301 accccgacgc cgcggcgggc ctgccctcgg cctcggctgc cgctgtcagc gccgacttca
      361 tgagcaacct gagcttgctg gaggagagcg aggacttccc gcaggcgccc ggctccgtgg
      421 cggcggcggt ggcggcggcg gccgccgcgg ccgccaccgg ggggctgtgc ggggacttcc
      481 agggcccgga ggcgggctgc ctgcacccag
//
