LOCUS       sequence-13172-e        3354 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1233-HQ858816
FEATURES             Location/Qualifiers
     source          1..3354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(911..1010)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1027..1046)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1028..1046)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1051..1067)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1051..1067)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1169..1188)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1180..1989
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1247..1266)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2136..2724
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2625..2644
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2878..2895
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3054..3081)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3173..3259)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3308..3330
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3322..3339
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3323..3339
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atgaggaaga agctgggcac ccgcttcccc gcggcacgga tcaaaaagat catgcaggct
      181 gatgaggacg ttggcaagat tgcactagct gtacctgttt tagtatcgag ggcccttgaa
      241 ttgtttttgc aagatttaat tgaccgaact tacgaaatta cactgcaaag tggtgcaaag
      301 acattgaatt ccttccacct aaagcaatgt gtgaggaggt acagttcttt tgacttccta
      361 actgaggttg tcaacaaggt accggacctc ggtggcgctg actcatgtgg agatgataga
      421 gcattaccca gaagaagaaa agccttgcca aatggaagtg acccagagaa tgaggaatct
      481 cgatcaagca aaatggccgt aagaagtgca aatatcagtc ccagaggacg tgggagaggt
      541 cgaggtagag ggcgaggacg accacccacc aagcggaagg aagttggtta tgtacaattt
      601 gaggatgaga gcagcatgtt tgctgatcag ggcgaagcct taccaggaga ggagacggtt
      661 ccagagacca tccatggcac cgagagcgta cctccaagca cacaccctcc agcagaagcc
      721 ccatcggcag cagagatacc agctccaaat ccaaaggtgg aagaagcgaa aaacgacgac
      781 catcagccgg attggcctat gccagatgcg attggaaaca tcggtgtcgg accatccggt
      841 tttggacatc ttacggtgca agttgacgag gatgaggact acgacaacga ggataagggt
      901 gggcgcgccg acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
      961 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
     1021 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     1081 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
     1141 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
     1201 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     1261 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
     1321 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     1381 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     1441 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta
     1501 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     1561 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     1621 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     1681 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
     1741 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     1801 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     1861 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     1921 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     1981 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     2041 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga
     2101 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2161 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2221 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2281 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2341 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2401 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2461 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     2521 cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     2581 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     2641 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     2701 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     2761 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     2821 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     2881 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     2941 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     3001 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
     3061 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
     3121 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
     3181 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
     3241 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
     3301 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccc
//
