LOCUS       sequence-131740-        3865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic gene corresponding to Synechocystis PCC6803 orf sll1489.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Kappock
  TITLE     Synechocystis PCC6803 CpmA
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK588
FEATURES             Location/Qualifiers
     source          1..3865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     445..464
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        445..463
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    464..488
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             503..525
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             536..553
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             578..598
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1442..1459
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1512..1530)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1526..1573
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1644..1660)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1644..1660)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1657..1679)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1668..1684
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1692..1698,1699..1716,1717..1722))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1737..1758
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1875..1892)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2046..2634)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2126..2145)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2805..3596,3597..3665))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3428..3447
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3666..3770)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3838..3856
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tgacccaatg aaattaatac gactcactat aggggaattg tgagcggata
      481 acaattcccc tctagaaata attttgttta actttaagaa ggagatatac atatgcacca
      541 tcatcatcat cattcaagcg gcgtggatct gggcaccgaa aatctgtact ttcagtctgt
      601 gtctggcccg gtcttccagt ttagtgcacc gatgacgccg gatagcctgc aacagctgct
      661 gacggcaatc gcaagcggtc acattagctc tcaagaaggc tttgaacagc tgaaacatct
      721 gagttttcag gcgatcgatg acttcgccaa agtggatcac cagcgtcaac tgcgcaccgg
      781 tttcccggaa gtggtttggg gcccgggtaa aacgccggaa cagattgaac aaattatcca
      841 ggtcctggca gtgcataacc cggtcgtgct ggttacccgt gtcgaaccgg aagtggctga
      901 tctgctgggc gaccgtattc cgctgctgca atattacccg caggcacgca tctgcgctct
      961 ggttcaaacc ccgctgcaga ttaaatattc aggcaccatc ggtgttctgt cggccggtac
     1021 ggcagatctg ccggtcgcag aagaagcggc cattacggcc agcctgtgtg gttttaaagt
     1081 tgaaaaactg tgggatgttg gcgtcgcagg gatccatcgt ctgctgtctc accgcgaact
     1141 gattcaggcc atggacgtgc tgatcgttgt cgcaggcatg gaaggtgctc tgccgtccgt
     1201 ggttgcaggc ctggttgatt gcccggtgat cggtgttccg accagtgtcg gctacggcac
     1261 gtcctttggc ggtgtggcac cgctgctgac catgctgaat agctgtgctg tgggcgttgg
     1321 tgtcgtgaac attgacaatg gcttcggtgc agctatgctg gccggtcaga tcctgcgtac
     1381 cgttgaccgt ctgcaaagta aatccacgat ggcgaatggc ggccagggcg gctgactcga
     1441 gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt
     1501 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
     1561 gaggggtttt ttgaattcat tgggtcatcg gatcccgggc ccgtcgactg cagaggcctg
     1621 catgcaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     1681 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     1741 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     1801 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     1861 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     1921 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     1981 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     2041 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2101 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2161 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     2221 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     2281 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     2341 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     2401 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     2461 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     2521 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     2581 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     2641 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     2701 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     2761 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     2821 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     2881 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     2941 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     3001 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     3061 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     3121 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     3181 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     3241 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     3301 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     3361 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     3421 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     3481 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     3541 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     3601 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     3661 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     3721 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     3781 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     3841 aggcgtatca cgaggccctt tcgtc
//
