LOCUS       sequence-13175-e        3387 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1236-HQ858819
FEATURES             Location/Qualifiers
     source          1..3387
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(944..1043)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1060..1079)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1061..1079)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1084..1100)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1084..1100)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1202..1221)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1213..2022
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1280..1299)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2169..2757
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2658..2677
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2911..2928
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3087..3114)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3206..3292)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3341..3363
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3355..3372
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3356..3372
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atgaaggtgc agtgcgacgt gtgcgcggcc gaggcggcgt cggtgttctg ctgcgccgac
      181 gaggccgcgc tgtgcgacgc gtgcgaccac cgggtgcacc gggccaacaa gctcgccggg
      241 aagcaccgcc ggttctcgct gctcaacccc tcggcgtccg gccgctcgcc gacatcgacg
      301 acggcgccgc tctgcgacat ctgccaggag aagaggggtt tcctgttctg caaggaggac
      361 cgggcgatcc tgtgccgcga gtgcgacgtg ccggtgcaca cggcgagcga gctcaccatg
      421 cgccacagcc ggtacctcct caccggcgtg cggctctcct cggagcctgc cgcgtccccg
      481 gcgccgccgt cggaggagga gaacagcagc agcttctgct gcagcgccga cgacgccgtg
      541 ccggccccgg cggcgcccgc cacgagccac ggcgggagca gcggcagcag cagcatctcc
      601 gagtacctca ccacgctgcc cgggtggcac gtcgaggact tcctcgtcga cgacgccact
      661 gccgaggccg ccgccgccgc cgccgccacc tcttccggca tctccgcgaa cgggccgtgt
      721 cagggggtaa cacggatcgg agggctgcaa gaatccgccg gctaccctgc gtggatggcg
      781 cagcagcagc tgtgctgcga cggcctcgtc gccggcgacg cgtcgccggc tagccgggag
      841 cggtgggtgc cgcagatgta cgcggatcag cttgccgccg gcagcaagag atccaggacg
      901 tccactgctt cttcctactc ctactggaag ggtgggcgcg ccgacccagc tttcttgtac
      961 aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc
     1021 agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac
     1081 atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt
     1141 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat
     1201 acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac
     1261 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     1321 acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     1381 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     1441 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     1501 actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     1561 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     1621 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     1681 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     1741 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     1801 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     1861 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     1921 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     1981 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
     2041 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa
     2101 tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa
     2161 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     2221 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     2281 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     2341 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     2401 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     2461 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     2521 cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt
     2581 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     2641 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     2701 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     2761 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     2821 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     2881 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     2941 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     3001 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct
     3061 agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag
     3121 tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca
     3181 caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac
     3241 aacagataaa acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg
     3301 cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgttgtaaa
     3361 acgacggcca gtcttaagct cgggccc
//
