LOCUS       sequence-13176-e        3837 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1237-HQ858820
FEATURES             Location/Qualifiers
     source          1..3837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1394..1493)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1510..1529)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1511..1529)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1534..1550)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1534..1550)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1652..1671)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1663..2472
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1730..1749)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2619..3207
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3108..3127
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3361..3378
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3537..3564)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3656..3742)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3791..3813
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3805..3822
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3806..3822
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atggccaagc gacgcagcaa cggcgagacc gccgccgcga gcagcgacga ctctagctcc
      181 ggcgtctgcg gcggcggcgg cggcggtgag gttgagccga ggcggcggca gaagcggccg
      241 cggaggagcg ccccgcggga ttgcccctcc cagcgcagct ccgcgttccg cggcgtcaca
      301 cggcaccggt ggacggggcg gttcgaggcg catctctggg acaagaacac ctggaacgag
      361 tcgcagagca agaagggcag acaagtttac ctcggggctt acgacggcga ggaagcggcg
      421 gcgcgcgcct acgacctcgc cgcattgaag tactggggcc acgacaccgt cctcaacttc
      481 cctctgtcaa catatgacga ggaattgaag gaaatggagg ggcagtccag ggaagagtac
      541 atcggatcgc tccggaggaa gagcagtggc ttctcaagag gggtgtccaa gtacagagga
      601 gttgcaaggc atcatcacaa cggcaaatgg gaggctcgga ttgggcgtgt gttcggcaac
      661 aaatacctct acctaggtac ttatgcaaca caagaggagg cggccgtggc gtacgacatc
      721 gcggcgatcg agcaccgcgg cctcaacgcc gtcaccaact tcgacatcaa tctctacatc
      781 aggtggtacc acggctcttg ccgctccagc agcgccgccg ccgccaccac catcgaagac
      841 gatgatttcg ccgaagccat cgccgccgcg ttgcaaggcg tcgacgagca gccgtcgtcg
      901 tcgccggcga cgacgcgcca gctgcaaacc gcggacgacg acgacgacga cctcgtggcg
      961 cagctcccgc cccagctgag gccgctggct cgcgcggcgt ccacctcccc gatcggactg
     1021 ctgctgcggt cgcccaagtt caaggagatc atcgagcagg cggcggccgc ggcggcgtcg
     1081 tcctctggta gcagcagtag cagcagcaca gactcacctt cttcttcgtc gtcgtcatcg
     1141 ctgtcgccgt cgccattgcc atcgccgccg ccgcagcagc agccaaccgt accgaaggac
     1201 gaccagtaca acgtcgacat gtcgtcggtg gcggcggcga ggtgcagctt cccggacgac
     1261 gtgcagacgt acttcgggct ggacgacgac ggcttcgggt acccggaggt ggacacgttc
     1321 ttgttcgggg atttgggcgc gtacgcggcg cccatgtttc agttcgagct cgacgtcaag
     1381 ggtgggcgcg ccgacccagc tttcttgtac aaagttggca ttataagaaa gcattgctta
     1441 tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca
     1501 gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg
     1561 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca
     1621 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
     1681 gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
     1741 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat
     1801 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
     1861 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
     1921 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag
     1981 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
     2041 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt
     2101 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
     2161 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc
     2221 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
     2281 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
     2341 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
     2401 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
     2461 gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact
     2521 tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac
     2581 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2641 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     2701 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2761 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2821 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2881 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2941 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     3001 cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     3061 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     3121 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     3181 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     3241 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     3301 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     3361 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     3421 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     3481 gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa
     3541 ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc
     3601 ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg
     3661 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg
     3721 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca
     3781 tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccc
//
