LOCUS       sequence-131760-         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pFOSGE_COF1B03
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    57..81
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(571..670)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(687..706)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(688..706)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(711..727)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(711..727)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa
       61 ctttgtacaa aaaagcaggc ttcatgtcca tggaaatacg catcaagtgc cgcgagatgt
      121 tggcaaccgc attgaaatcg ggcaacatgc caccaggatg tggcgatccg gatgacatgg
      181 ccgctaaact ggaggacgca atttacggcg acttgaacgg ctgcaaggtg aagtacaaaa
      241 atcgcatacg ttcgcgtttg gccaacttgc gcgatccgaa gaatccagag ctgcgccaga
      301 agttcctatt aggtcaaata acgcccgaag agttatccaa gatgacgccc gaggagatgg
      361 ccagcgatga tatgaagcag atgcgccaga agtacgttca ggactcgatc aatgcggcac
      421 agatggccaa ggttcagggc accaagacgg atcagttcaa atgcgaacgc tgcgacaaac
      481 gcaactgcag ccaattgcat atccgtgatg gtgacgagcc cattatcacc tttgtgatat
      541 gcgacgagtg cggcaaccgc tggaagagcg acccagcttt cttgtacaaa gttggcatta
      601 taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
      661 tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg
      721 tttcctggca gctctgg
//
