LOCUS       sequence-131770-         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB111
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(38..162)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(184..529)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(221..241)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 agcctggggt gcctaatgcg gcccctcgag gggccgccat agtgactgga tatgttgtgt
       61 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
      121 atcattttac gtttctcgtt cagctttttt gtacaaactt gttgataact ctagagtccc
      181 ccgtgttctc tccaaatgaa atgaacttcc ttatatagag gaagggtctt gcgaaggata
      241 gtgggattgt gcgtcatccc ttacgtcagt ggagatatca catcaatcca cttgctttga
      301 agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt
      361 gggaccactg tcggcagagg catcttcaac gatggccttt cctttatcgc aatgatggca
      421 tttgtaggag ccaccttcct tttccactat cttcacaata aagtgacaga tagctgggca
      481 atggaatccg aggaggtttc cggatattac cctttgttga aaagtctcaa ttgccctttg
      541 gtcttctgag actgtatctt tgatattttt ggagtagaca agtgtgtcgt gctccaccat
      601 gttgacgaag attttcttct tgtcattgag tcgtaagaga ctctgtatga actgttcgcc
      661 agtctttacg gcgagttctg ttaggtcctc tatttgaatc tttgactcca tggcctttga
      721 ttcagtggga actacctttt tagagactcc
//
