LOCUS       sequence-131774-         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB119
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(45..169)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(191..536)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(228..248)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 agtgtaaagc ctggggtgcc taatgcggcc cctcgagggg ccgccatagt gactggatat
       61 gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga
      121 tatttatatc attttacgtt tctcgttcag cttttttgta caaacttgtt gataactcta
      181 gagtcccccg tgttctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgcg
      241 aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt
      301 gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc
      361 catctttggg accactgtcg gcagaggcat cttcaacgat ggcctttcct ttatcgcaat
      421 gatggcattt gtaggagcca ccttcctttt ccactatctt cacaataaag tgacagatag
      481 ctgggcaatg gaatccgagg aggtttccgg atattaccct ttgttgaaaa gtctcaattg
      541 ccctttggtc ttctgagact gtatctttga tatttttgga gtagacaagt gtgtcgtgct
      601 ccaccatgtt gacgaagatt ttcttcttgt cattgagtcg taagagactc tgtatgaact
      661 gttcgccagt ctttacggcg agttctgtta ggtcctctat ttgaatcttt gactccatgg
      721 cctttgattc agtgggaact acctttt
//
