LOCUS       sequence-13178-e        3006 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1320-HQ858822
FEATURES             Location/Qualifiers
     source          1..3006
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(563..662)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(679..698)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(680..698)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(703..719)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(703..719)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(821..840)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             832..1641
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(899..918)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1788..2376
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2277..2296
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2530..2547
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2706..2733)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2825..2911)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2960..2982
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2974..2991
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2975..2991
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atggccggga ttggtccgat caggcaggac tgggagccgg tggtggtgcg gaagaaggcg
      181 cccaccgccg ccgccaagaa ggatgagaag gccgtcaacg ccgcccgccg ctccggcgcc
      241 gagatcgaga ccatgaagaa gtataacgct ggaacaaaca aggcggcgtc cagtggcaca
      301 tccctcaaca ccaagcggct ggatgacgac accgagagcc ttgcccatga gcgtgtctca
      361 agtgacctga agaaaaacct catgcaagca aggctggaca agaagatgac ccaggcacag
      421 cttgcacaga tgatcaatga gaagccccag gtgatccagg agtacgagtc aggtaaagct
      481 attccgaacc agcagatcat cgggaagctt gaaagggctc ttggaacaaa gctgcgcggc
      541 aagaataagg gtgggcgcgc cgacccagct ttcttgtaca aagttggcat tataagaaag
      601 cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
      661 tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
      721 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
      781 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
      841 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg
      901 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg
      961 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     1021 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     1081 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca
     1141 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     1201 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     1261 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     1321 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     1381 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     1441 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     1501 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     1561 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     1621 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
     1681 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg
     1741 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     1801 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1861 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1921 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1981 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     2041 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     2101 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     2161 agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac
     2221 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     2281 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     2341 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     2401 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     2461 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     2521 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     2581 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     2641 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa
     2701 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
     2761 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
     2821 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     2881 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
     2941 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc
     3001 gggccc
//
