LOCUS       sequence-13179-e        3240 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1321-HQ858823
FEATURES             Location/Qualifiers
     source          1..3240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(797..896)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(913..932)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(914..932)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(937..953)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(937..953)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1055..1074)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1066..1875
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1133..1152)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2022..2610
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2511..2530
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2764..2781
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2940..2967)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3059..3145)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3194..3216
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3208..3225
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3209..3225
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atgacggccg ccctgcaagc gctgctcgac ccgacggcgc tgtccttggg cctgcccacg
      181 ccggcgatca acaaggagga gtacctcgcc atctgcctcg ccgcgctcgc ctgcacgcgg
      241 gcggggaagg ccctggtggg agtgggaggc cagcagcagg tgcaggcctg caataagtgg
      301 ctgtgcccag cgcccgcggc gcctgaggag ctccgcttcc ggtgcaccgt ctgcgggaag
      361 gccttcgcct cgtaccaggc gctcggcgga cacaagtcca gccaccggaa gccgccttcc
      421 ccgggagacc actacggcgc cgccgccgcg gcgcagcagc tggcatccgc tggtgactcg
      481 aaggaggact cggcgtcgtc agcggccggg agcactggcc cgcaccggtg caccatctgc
      541 aggaggagct tcgcgacggg gcaggcgctc ggcggccaca agcgctgcca ctactgggac
      601 ggcacgtcgg tgtccgtctc cgtctcggcg tccgcgtcgg ccgcctcgtc ggccgtcagg
      661 aacttcgacc tcaacctgat gccgctgccg gagagcacag ccgccgccgg gatcaagagg
      721 tgggcggagg aggaggaggt gcagagtcca ctgccggtca agaagctcag gatgtccaac
      781 aagggtgggc gcgccgaccc agctttcttg tacaaagttg gcattataag aaagcattgc
      841 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
      901 ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
      961 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
     1021 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     1081 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     1141 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
     1201 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     1261 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     1321 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc
     1381 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     1441 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     1501 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     1561 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     1621 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     1681 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     1741 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     1801 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     1861 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     1921 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc
     1981 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2041 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2101 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2161 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2221 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2281 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2341 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2401 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2461 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2521 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2581 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2641 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     2701 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     2761 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2821 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2881 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa
     2941 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     3001 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     3061 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     3121 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta
     3181 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
//
