LOCUS       sequence-131791-       12457 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12457)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 12457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB214
FEATURES             Location/Qualifiers
     source          1..12457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1179..1303
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1341..1346,1347..1364,1365..1371)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1425..2084
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1491..1510)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2426..2731
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2610..2629
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2772..2896)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3798..4050
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4084..4100)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4084..4100)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4097..4119)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4108..4124
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4132..4138,4139..4156,4157..4162))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4177..4198
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4283..4455
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4512..5045)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MVRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(5108..5784)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    5979..6003
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6524..7315
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7561..8149
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8050..8069
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8303..8320
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8335..8475
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8401..8420)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8561..8583
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8819..9013
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9079..10152)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10581..11210)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctgcgagggg
     1321 ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt
     1381 tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
     1441 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
     1501 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
     1561 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
     1621 tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg
     1681 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
     1741 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     1801 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
     1861 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     1921 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
     1981 cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta
     2041 atgaattaca acagtactgc gatgagtggc agggcggggc gtaaagatct ggatccggct
     2101 tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat
     2161 atactgatat gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac
     2221 agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc
     2281 ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa
     2341 gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt
     2401 gctgacgaga acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag
     2461 ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat
     2521 ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtcccccgtg aactttaccc
     2581 ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc
     2641 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
     2701 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
     2761 tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt
     2821 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt
     2881 tcttgtacaa agtggttgat aacagcatgg ctagtgaagc tcgaaaaaca aagaaaaaaa
     2941 tcaaagggat tcagcaagcc actgcaggag tctcacaaga cacttcggaa aatcctaaca
     3001 aaacaatagt tcctgcagca ttaccacagc tcacccctac cttggtgtca ctgctggagg
     3061 tgattgaacc cgaggtgttg tatgcaggat atgatagctc tgttccagat tcagcatgga
     3121 gaattatgac cacactcaac atgttaggtg ggcgtcaagt gattgcagca gtgaaatggg
     3181 caaaggcgat accaggcttc agaaacttac acctggatga ccaaatgacc ctgctacagt
     3241 actcatggat gtttctcatg gcatttgccc tgggttggag atcatacaga caatcaagtg
     3301 gaaacctgct ctgctttgct cctgatctga ttattaatga gcagagaatg tctctaccct
     3361 gcatgtatga ccaatgtaaa cacatgctgt ttgtctcctc tgaattacaa agattgcagg
     3421 tatcctatga agagtatctc tgtatgaaaa ccttactgct tctctcctca gttcctaagg
     3481 aaggtctgaa gagccaagag ttatttgatg agattcgaat gacttatatc aaagagctag
     3541 gaaaagccat cgtcaaaagg gaagggaact ccagtcagaa ctggcaacgg ttttaccaac
     3601 tgacaaaact tctggactcc atgcatgagg tggttgagaa tctccttacc tactgcttcc
     3661 agacattttt ggataagacc atgagtattg aattcccaga gatgttagct gaaatcatca
     3721 ctaatcagat accaaaatat tcaaatggaa atatcaagaa gctgctgttt catcaaaaat
     3781 agagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc
     3841 ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa
     3901 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc
     3961 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat
     4021 cgcgcgcggt gtcatctatg ttactagatc gggaattggt tccggaacca attcgtaatc
     4081 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     4141 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattagg
     4201 ctgaattagg cgcgcctatt tctgaattca acacattgcg gacgttttta atgtactgaa
     4261 ttaacgccga attaattcgg gggatctgga ttttagtact ggattttggt tttaggaatt
     4321 agattttatt gatagaagta ttttacaaat acaaatacat actaagggtt tcttatatgc
     4381 tcaacacatg agcgaaaccc tataggaacc ctaattccct tatctgggaa ctactcacac
     4441 attattatgg agaaactcga gcttgtcgat cgactctagg ccgggaagcc gatctcggct
     4501 tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt
     4561 atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag
     4621 atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag cgcggtggca
     4681 atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat ctcactacgc
     4741 ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg
     4801 aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta atcggagtcc
     4861 ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag atcaagagca
     4921 gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat gcccatactt
     4981 gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctgcgt
     5041 accatggtcg atcgacagat ctgcgaaagc tcgagagaga tagatttgta gagagagact
     5101 ggtgatttca gcgtgtcctc tccaaatgaa atgaacttcc ttatatagag gaagggtctt
     5161 gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt ggagatatca catcaatcca
     5221 cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg
     5281 gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc
     5341 aatgatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga
     5401 tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa
     5461 tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt
     5521 gctccaccat gttcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt
     5581 tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct
     5641 tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct
     5701 actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat
     5761 attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata
     5821 ttcttggagt agacgagagt gtcgtgctcc accatgttgg caagctgctc tagccaatac
     5881 gcaaaccgaa ttcagaaata aattcagcct aattcggcgt taattcagta cattaaaaac
     5941 gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg
     6001 ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca
     6061 ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg
     6121 ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga
     6181 tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc
     6241 aattcgggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta
     6301 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
     6361 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
     6421 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     6481 tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatggggg aagcggtgat
     6541 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     6601 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     6661 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     6721 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     6781 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     6841 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     6901 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     6961 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     7021 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     7081 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     7141 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     7201 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     7261 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct
     7321 agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca agcgttagat
     7381 gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt
     7441 taagcgtgca taataagccc tacacaaatt gggagatata tcatgcatga ccaaaatccc
     7501 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     7561 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7621 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     7681 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     7741 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     7801 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7861 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7921 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7981 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     8041 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     8101 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     8161 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     8221 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     8281 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
     8341 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     8401 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
     8461 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     8521 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     8581 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
     8641 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
     8701 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
     8761 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
     8821 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
     8881 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
     8941 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
     9001 cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc
     9061 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
     9121 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
     9181 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
     9241 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
     9301 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
     9361 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
     9421 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
     9481 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
     9541 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
     9601 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
     9661 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
     9721 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
     9781 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
     9841 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
     9901 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
     9961 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
    10021 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
    10081 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
    10141 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
    10201 acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
    10261 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
    10321 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
    10381 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
    10441 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
    10501 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
    10561 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
    10621 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
    10681 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
    10741 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
    10801 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
    10861 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
    10921 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
    10981 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
    11041 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
    11101 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
    11161 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
    11221 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
    11281 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
    11341 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
    11401 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
    11461 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
    11521 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
    11581 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
    11641 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
    11701 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
    11761 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
    11821 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
    11881 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
    11941 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
    12001 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
    12061 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
    12121 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
    12181 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
    12241 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
    12301 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
    12361 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
    12421 ttggcatgca catacaaatg gacgaacgga taaacct
//
