LOCUS       sequence-131796-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S,
            Callaway EM
  TITLE     Short Promoters in Viral Vectors Drive Selective Expression in 
            Mammalian Inhibitory Neurons, but do not Restrict Activity to 
            Specific Inhibitory Cell-Types.
  JOURNAL   Front Neural Circuits. 2009 Nov 9;3:19. doi: 
            10.3389/neuro.04.019.2009. eCollection 2009.
  PUBMED    19949461
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Neural Circuits."; date: "2009-11-9"; volume: "3:19. doi"; pages: " 
            10.3389/neuro.04.019.2009. eCollection 2009"
COMMENT     Sequence Label: pAAV-fSST-RFP
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(58..735)
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(120..143)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     535..555
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     regulatory      732..741
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    749..801
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 ctatagaata gggccgtttc ccgtacggtt taaacagctc caccgcggtg gcggccgcta
       61 caggaacagg tggtggcggc cctcggtgcg ctcgtactgc tccacgatgg tgtagtcctc
      121 gttgtgggag gtgatgtcca gcttggcgtc cacgtagtag tagccgggca gctgcacggg
      181 cttcttggcc atgtagatgg acttgaactc caccaggtag tggccgccgt ccttcagctt
      241 cagggccttg tgggtctcgc ccttcagcac gccgtcgcgg gggtacaggc gctcggtgga
      301 ggcctcccag cccatggtct tcttctgcat cacggggccg tcggagggga agttcacgcc
      361 gatgaacttc accttgtaga tgaagcagcc gtcctgcagg gaggagtcct gggtcacggt
      421 cgccacgccg ccgtcctcga agttcatcac gcgctcccac ttgaagccct cggggaagga
      481 cagcttcttg tagtcgggga tgtcggcggg gtgcttcacg tacaccttgg agccgtactg
      541 gaactggggg gacaggatgt cccaggcgaa gggcaggggg ccgcccttgg tcaccttcag
      601 cttcacggtg ttgtggccct cgtaggggcg gccctcgccc tcgccctcga tctcgaactc
      661 gtggccgttc acggtgccct ccatgcgcac cttgaagcgc atgaactcgg tgatgacgtt
      721 ctcggaggag gccatggtgg cgaccggtgg atcccgggcc cgcggtaccg tcgactgcag
      781 aattcgaagc ttgagctcga gggggggccc ggtacggtca ccggcccagg cggccagatc
      841 ttcgaattca cgtgctttgc caaagtgatg ggccagcaca cagaccagca cgttgccc
//
