LOCUS       sequence-13180-e        3453 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1322-HQ858824
FEATURES             Location/Qualifiers
     source          1..3453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1010..1109)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1126..1145)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1127..1145)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1150..1166)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1150..1166)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1268..1287)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1279..2088
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1346..1365)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2235..2823
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2724..2743
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2977..2994
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3153..3180)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3272..3358)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3407..3429
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3421..3438
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3422..3438
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atggctgcag ctatagatct gtcaggggag gagctgatga gagcactcga gccttttatc
      181 cgagatgcct ccggctcgcc tccggtttgt tcccagttta gtcccacctc gccattctcc
      241 ttcccgcacg cgttcgcgta cggtggcggg ctggcccagc agccagagct cagcccggcc
      301 cagatgcact acatccaggc ccgcctccac ctccagcggc aggcggcgca ggccggcccg
      361 ctcggcccgc gcgcgcagcc gatgaaggcg tcgtcgtcgt cggcttcggc ggcgggggcg
      421 gcggcgacgc cgccgcggcc gcagaagctg taccgcggcg tgcggcagcg gcactggggg
      481 aagtgggtgg cggagatccg cctcccgcgg aaccgcacgc ggctctggct cggcaccttc
      541 gacacagccg aggaggcggc gcttgcctac gaccaggcgg cgtaccgcct ccgcggcgac
      601 gcggcgcggc tcaacttccc cgacaacgcc gcctcccgcg gcccgctcca cgcctccgtc
      661 gacgccaagc tccagacgct ctgccagaac atcgccgccg ccaagaacgc caagaagtcc
      721 tctgtctccg cctccgccgc cgcaacatcc tccgcgccca ccagcaactg ctcgtcgccg
      781 tcctccgacg acgcgtcgtc ctgcctggag tccgccgact cgtcgccctc cctgtcgccg
      841 tcctccgccg ccaccacggc cgagacgccg gcgaccgtgc cggagatgca gcagctcgac
      901 ttcagcgagg cgccgtggga cgaggccgcc gccttcgccc tcaccaagta cccgtcctac
      961 gagatcgact gggactccct cctcgccgcc aataagggtg ggcgcgccga cccagctttc
     1021 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
     1081 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg
     1141 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
     1201 tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac
     1261 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat
     1321 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     1381 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     1441 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     1501 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     1561 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca
     1621 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     1681 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     1741 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     1801 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     1861 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     1921 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     1981 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     2041 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     2101 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga
     2161 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag
     2221 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2281 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2341 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     2401 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2461 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2521 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2581 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc
     2641 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2701 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2761 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2821 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2881 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     2941 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     3001 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     3061 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt
     3121 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct
     3181 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt
     3241 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg
     3301 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat
     3361 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt
     3421 tgtaaaacga cggccagtct taagctcggg ccc
//
