LOCUS       sequence-131805-       13935 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13935)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 13935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB322
FEATURES             Location/Qualifiers
     source          1..13935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1179..1303
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1340..1345,1346..1363,1364..1370)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1424..2083
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1490..1509)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2425..2730
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2609..2628
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2771..2895)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3842..4094
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4128..4144)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4128..4144)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4141..4163)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4152..4168
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4176..4182,4183..4200,4201..4206))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4221..4242
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4327..4502
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(6586..7262)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    7457..7481
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8002..8793
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9039..9627
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9528..9547
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9781..9798
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9813..9953
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9879..9898)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10039..10061
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10297..10491
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10557..11630)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12059..12688)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatcgttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
     1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1381 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1981 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     2041 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     2101 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2161 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     2221 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2281 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2341 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2401 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2461 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2521 gtgatccccc tggccagtgc acgtctgctg tcagataaag tcccccgtga actttacccg
     2581 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2641 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2701 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2761 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2821 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2881 cttgtacaaa gtggttgata acagcttgga tttggatttg gagttgcgct tgggattcgc
     2941 ctccgccgga atggctagtg aagctcgaaa aacaaagaaa aaaatcaaag ggattcagca
     3001 agccactgca ggagtctcac aagacacttc ggaaaatcct aacaaaacaa tagttcctgc
     3061 agcattacca cagctcaccc ctaccttggt gtcactgctg gaggtgattg aacccgaggt
     3121 gttgtatgca ggatatgata gctctgttcc agattcagca tggagaatta tgaccacact
     3181 caacatgtta ggtgggcgtc aagtgattgc agcagtgaaa tgggcaaagg cgataccagg
     3241 cttcagaaac ttacacctgg atgaccaaat gaccctgcta cagtactcat ggatgtttct
     3301 catggcattt gccctgggtt ggagatcata cagacaatca agtggaaacc tgctctgctt
     3361 tgctcctgat ctgattatta atgagcagag aatgtctcta ccctgcatgt atgaccaatg
     3421 taaacacatg ctgtttgtct cctctgaatt acaaagattg caggtatcct atgaagagta
     3481 tctctgtatg aaaaccttac tgcttctctc ctcagttcct aaggaaggtc tgaagagcca
     3541 agagttattt gatgagattc gaatgactta tatcaaagag ctaggaaaag ccatcgtcaa
     3601 aagggaaggg aactccagtc agaactggca acggttttac caactgacaa aacttctgga
     3661 ctccatgcat gaggtggttg agaatctcct tacctactgc ttccagacat ttttggataa
     3721 gaccatgagt attgaattcc cagagatgtt agctgaaatc atcactaatc agataccaaa
     3781 atattcaaat ggaaatatca agaagctgct gtttcatcaa aaatagagct cgaatttccc
     3841 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     3901 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     3961 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4021 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4081 tatgttacta gatcgggaat tggttccgga accaattcgt aatcatggtc atagctgttt
     4141 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     4201 tgtaaagcct ggggtgccta atgagtgagc taactcacat taggctgaat taggcgcgcc
     4261 tatttctgaa ttcaacacat tgcggacgtt tttaatgtac tgaattaacg ccgaattaat
     4321 tcgggggatc tggattttag tactggattt tggttttagg aattagaaat tttattgata
     4381 gaagtatttt acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg
     4441 aaaccctata ggaaccctaa attcccttat ctgggaacta ctcacacatt attatggaga
     4501 aactcgaagt tagtatatag tccttccatc accctctgtg ataatctcct taaaacttgc
     4561 tcccccagga atcataggca gaacgtgctc ctggtgggga actatcacat ccagtaaata
     4621 aggaccggga gaatctagca tagtttgaat tgctgctctt agttcatcct tcttcaccac
     4681 acgcgcattt ggcaccttga aaccgtctgc aactgtacag aagtcggggt agatctgctc
     4741 ctcgtgatct ggatcaccta agtaagtatg tgctctgttg gccttgtaga atctatcctc
     4801 caattgtacg accattccta gatgttggtt gtttaagatc atgatcttca cgggtaactt
     4861 ctccacaaaa attgtagcca actcctgtac gttcatcaca aagctaccat cgccatcgat
     4921 gtcgatcaca ggaatatctg gttgagttgc ggcagccccc aaagcagaag gaagtccgaa
     4981 tcccatggct ccaagacctc cagatgtcaa ccaacgccgc ggttcgttgt acttgaacca
     5041 ctgggctgcc cacatttgat gttggccaac accagtagat atgatggcat ttccaccagt
     5101 gagttcgtac agcgtctgta tggcagattg aggagcaatg gcatcctcga atacgggaaa
     5161 cttcattggc catttctgct tttgttcttc tagttctgcc acccagttgg agaagtcgaa
     5221 agtcctcttt tgactcttca agagggcatt gatttcactt agcgcaggtt tcacatcagc
     5281 acacatggac acatggggct gtttgttctt tccaatttca gcagggtcaa tatcgatatg
     5341 aacgatgctg gacctgctag caaaagtctc cagtttacca gtcactcgat cgtcgaaacg
     5401 cactccaaat gccaacaaca gatctgcttg gtccacggcg tagttggcgt aaacagttcc
     5461 atgcattcca agcattttca aacttaatgg atcagactcg ggatacgctc ccaaacccat
     5521 taaagtcgat gtgataggaa ttccggtaag ctcgacaaat tccctcaact cttcgctggc
     5581 gtgattgcaa cctcctccag aatacactac aggcttctta gaactagaaa tgagtcttat
     5641 aatctgctcg attaacgttg gttccggtgg ccgaggcagt cgttgtaagt agcccttcag
     5701 ttgcattggc tgagaccaat ctggaatagc catttgctgt tgcacgtcct tgggaatatc
     5761 taccaagact gggcccggcc tacctgaagc tgccaaaaag aaagcctccc tgattacgcg
     5821 agggatgtca tcaatgttca taacaaggta attgtgcttg gtgatcgatc tcgtaacctc
     5881 cacaattgga gtttcttgaa aagcatcggt accaatcatc ctactggata cttgacctgt
     5941 aatggccact agtggcacac tgtccaagag ggcatcagcg agcccggtaa caagattggt
     6001 cgctccagga ccggatgtgg ctatacaaac ccctactcgt ccagacgatt tagcataacc
     6061 ttcagcagcg aaaatctccc cctgctcgtg gcgacagaga atgttgcgga tgctgctgga
     6121 acgtgtaaga gcctggtgaa tctccataga cgctccgccg ggataagcga acaccgttct
     6181 aatcccttct cgctcaagag cctccaccaa aatgtcggct cctttgcggg gctcgtcatc
     6241 cttgtagcga gactgaaagt cggacggggt tggtgcaacg agtgacttcc cgctgctaag
     6301 ccgtggtcca ctcacatcga ctgcagactg gatcttctcg ctaacagctt gggctttcac
     6361 ttccagtgaa cgctcagtct tccgagaggt gttgtatgca tgtcccctca gagcggctcc
     6421 cgagaagaaa ccactcctca cctttagctc tttagctccc ttgcaacaag accttgccga
     6481 tggctgcacc ttctccaggc tcgctatgct caaaggcaca gctgcgcatc cactaattga
     6541 cgccatgctc gagagagata gatttgtaga gagagactgg tgatttcagc gtgtcctctc
     6601 caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt gggattgtgc
     6661 gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg
     6721 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc
     6781 ggcagaggca tcttgaacga tagcctttcc tttatcgcaa ttatggcatt tgtaggtgcc
     6841 accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag
     6901 gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac
     6961 tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt tcacatcaat
     7021 ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg
     7081 ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat
     7141 cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac
     7201 agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct
     7261 caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt
     7321 cgtgctccac catgttggca agctgctcta gccaatacgc aaaccgaatt cagaaataaa
     7381 ttcagcctaa ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt
     7441 gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc
     7501 ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg
     7561 gcgtcagcgg gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg
     7621 gaagaacggc aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg
     7681 ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcaa ttcgggcacg aacccagtgg
     7741 acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg
     7801 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
     7861 tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     7921 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
     7981 cctaaaacaa agttaaacat catgggggaa gcggtgatcg ccgaagtatc gactcaacta
     8041 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     8101 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     8161 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     8221 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     8281 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     8341 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     8401 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     8461 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     8521 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     8581 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     8641 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     8701 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg
     8761 aaaggcgaga tcaccaaggt agtcggcaaa taatgtctag ctagaaattc gttcaagccg
     8821 acgccgcttc gcggcgcggc ttaactcaag cgttagatgc actaagcaca taattgctca
     8881 cagccaaact atcaggtcaa gtctgctttt attattttta agcgtgcata ataagcccta
     8941 cacaaattgg gagatatatc atgcatgacc aaaatccctt aacgtgagtt ttcgttccac
     9001 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     9061 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     9121 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     9181 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     9241 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     9301 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     9361 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     9421 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     9481 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     9541 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     9601 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     9661 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     9721 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     9781 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat
     9841 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
     9901 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
     9961 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
    10021 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
    10081 aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga cggcgcggct
    10141 tgtccgcgcc ctggtagatt gcctggccgt aggccagcca tttttgagcg gccagcggcc
    10201 gcgataggcc gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct
    10261 ttttgcagct cttcggctgt gcgctggcca gacagttatg cacaggccag gcgggtttta
    10321 agagttttaa taagttttaa agagttttag gcggaaaaat cgcctttttt ctcttttata
    10381 tcagtcactt acatgtgtga ccggttccca atgtacggct ttgggttccc aatgtacggg
    10441 ttccggttcc caatgtacgg ctttgggttc ccaatgtacg tgctatccac aggaaagaga
    10501 ccttttcgac ctttttcccc tgctagggca atttgcccta gcatctgctc cgtacattag
    10561 gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag gatcgggcca
    10621 gcctgccccg cctcctcctt caaatcgtac tccggcaggt catttgaccc gatcagcttg
    10681 cgcacggtga aacagaactt cttgaactct ccggcgctgc cactgcgttc gtagatcgtc
    10741 ttgaacaacc atctggcttc tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa
    10801 cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc
    10861 ttgccttctg tgatctcgcg gtacatccaa tcagctagct cgatctcgat gtactccggc
    10921 cgcccggttt cgctctttac gatcttgtag cggctaatca aggcttcacc ctcggatacc
    10981 gtcaccaggc ggccgttctt ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt
    11041 aaccgaatgc aggtttctac caggtcgtct ttctgctttc cgccatcggc tcgccggcag
    11101 aacttgagta cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc tcccttccct
    11161 tcccggtatc ggttcatgga ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa
    11221 tcccacacac tggccatgcc ggccggccct gcggaaacct ctacgtgccc gtctggaagc
    11281 tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa aacggccacg
    11341 tccatgatgc tgcgactatc gcgggtgccc acgtcataga gcatcggaac gaaaaaatct
    11401 ggttgctcgt cgcccttggg cggcttccta atcgacggcg caccggctgc cggcggttgc
    11461 cgggattctt tgcggattcg atcagcggcc gcttgccacg attcaccggg gcgtgcttct
    11521 gcctcgatgc gttgccgctg ggcggcctgc gcggccttca acttctccac caggtcatca
    11581 cccagcgccg cgccgatttg taccgggccg gatggtttgc gaccgctcac gccgattcct
    11641 cgggcttggg ggttccagtg ccattgcagg gccggcagac aacccagccg cttacgcctg
    11701 gccaaccgcc cgttcctcca cacatggggc attccacggc gtcggtgcct ggttgttctt
    11761 gattttccat gccgcctcct ttagccgcta aaattcatct actcatttat tcatttgctc
    11821 atttactctg gtagctgcgc gatgtattca gatagcagct cggtaatggt cttgccttgg
    11881 cgtaccgcgt acatcttcag cttggtgtga tcctccgccg gcaactgaaa gttgacccgc
    11941 ttcatggctg gcgtgtctgc caggctggcc aacgttgcag ccttgctgct gcgtgcgctc
    12001 ggacggccgg cacttagcgt gtttgtgctt ttgctcattt tctctttacc tcattaactc
    12061 aaatgagttt tgatttaatt tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct
    12121 cgggttctga ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct
    12181 gcgtgatacg ggactcaaga atgggcagct cgtacccggc cagcgcctcg gcaacctcac
    12241 cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt agccttccat
    12301 ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt
    12361 aagggcttgg ctgcaccgga atcagcacga agtcggctgc cttgatcgcg gacacagcca
    12421 agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg atggccttca
    12481 cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt tagcggttga tcttcccgca
    12541 cggccgccca atcgcgggca ctgccctggg gatcggaatc gactaacaga acatcggccc
    12601 cggcgagttg cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct gacccgcctt
    12661 tctggttaag tacagcgata accttcatgc gttccccttg cgtatttgtt tatttactca
    12721 tcgcatcata tacgcagcga ccgcatgacg caagctgttt tactcaaata cacatcacct
    12781 ttttagacgg cggcgctcgg tttcttcagc ggccaagctg gccggccagg ccgccagctt
    12841 ggcatcagac aaaccggcca ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg
    12901 ctcgaacacg tacccggccg cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg
    12961 ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct cttggcgttc attctcggcg
    13021 gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc
    13081 tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt acagggtcga gcgatgcacg
    13141 ccaagcagtg cagccgcctc tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg
    13201 tgcgcgatct gtgccggggt gagggtaggg cgggggccaa acttcacgcc tcgggccttg
    13261 gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg
    13321 ccggcgaaca cggtcaacac catgcggccg gccggcgtgg tggtgtcggc ccacggctct
    13381 gccaggctac gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg
    13441 cgggtgctgc gggccaggcg gtctagcctg gtcactgtca caacgtcgcc agggcgtagg
    13501 tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa
    13561 aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg
    13621 tcggtgctga cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa
    13681 tgtctccggt tctagtcgca agtattctac tttatgcgac taaaacacgc gacaagaaaa
    13741 cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg
    13801 ttttcagaag acggctgcac tgaacgtcag aagccgactg cactatagca gcggaggggt
    13861 tggatcaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga
    13921 cgaacggata aacct
//
