LOCUS       sequence-131816-         456 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB336
FEATURES             Location/Qualifiers
     source          1..456
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(100..405)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(202..221)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg
       61 tcgacctgca gactggctgt gtataaggga gcctgacatt tatattcccc agaacatcag
      121 gttaatggcg tttttgatgt cattttcgcg gtggctgaga tcagccactt cttccccgat
      181 aacggagacc ggcacactgg ccatatcggt ggtcatcatg cgccagcttt catccccgat
      241 atgcaccacc gggtaaagtt cacgggagac tttatctgac agcagacgtg cactggccag
      301 ggggatcacc atccgtcgcc cgggcgtgtc aataatatca ctctgtacat ccacaaacag
      361 acgataacgg ctctctcttt tataggtgta aaccttaaac tgcatttcac cagcccctgt
      421 tctcgtcagc aaaagagccg ttcatttcaa taaacc
//
