LOCUS       sequence-131830-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-Mito-7
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..87
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      115..124
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             121..777
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 agatccgcta gcatgtccgt cctgacgccg ctgctgctgc ggggcttgac aggctcggcc
       61 cggcggctcc cagtgccgcg cgccaagatc cattcgttgg gggatccacc ggtcgccacc
      121 atggtgagtg tgattaaacc agagatgaag atgaggtact acatggacgg ctccgtcaat
      181 gggcatgagt tcacaattga aggtgaaggc acaggcagac cttacgaggg acatcaagag
      241 atgacactac gcgtcacaat ggccgagggc gggccaatgc ctttcgcgtt tgacttagtg
      301 tcacacgtgt tctgttacgg ccacagagta tttactaaat atccagaaga gataccagac
      361 tatttcaaac aagcatttcc tgaaggcctg tcatgggaaa ggtcgttgga gttcgaagat
      421 ggtgggtccg cttcagtcag tgcgcatata agccttagag gaaacacctt ctaccacaaa
      481 tccaaattta ctggggttaa ctttcctgcc gatggtccta tcatgcaaaa ccaaagtgtt
      541 gattgggagc catcaaccga gaaaattact gccagcgacg gagttctgaa gggtgatgtt
      601 acgatgtacc taaaacttga aggaggcggc aatcacaaat gccaaatgaa gactacttac
      661 aaggcggcaa aagagattct tgaaatgcca ggagaccatt acatcggcca tcgcctcgtc
      721 aggaaaaccg aaggcaacat tactgagcag gtagaagatg cagtagctca tagctaagcg
      781 gccgcgactc tagatcataa tcagccatac cacatttgta gaggtt
//
