LOCUS       sequence-131839-         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes PLK4 isoform 1 carrying S285A/T289A mutations 
            with an N-terminal EGFP tag and C-terminal 3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Sillibourne JE, Tack F, Vloemans N, Boeckx A, Thambirajah S, Bonnet 
            P, Ramaekers FC, Bornens M, Grand-Perret T
  TITLE     Autophosphorylation of polo-like kinase 4 and its role in centriole 
            duplication.
  JOURNAL   Mol Biol Cell. 2010 Feb 15;21(4):547-61. doi: 
            10.1091/mbc.E09-06-0505. Epub 2009 Dec 23.
  PUBMED    20032307
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E09-06-0505"; journalName: "Mol Biol Cell"; date: 
            "2010-02-15- 15"; volume: "21"; issue: "4"; pages: "547-61"
COMMENT     Sequence Label: pEGFP-C3-PLK4-S285A/T289A-3xFLAG
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atccttttat gtcccgaaat tcttcaacaa aaagtaaaga tttaggaact gtggaagact
       61 caattgatgc tgggcatgcc gcaatttcta ctgcaattac agcttcttcc agtaccagta
      121 taagtggtag tttatttgac aaaagaagac ttttgattgg tcagccactc ccaaataaaa
      181 tgactgtatt tccaaagaat aaaagttcaa ctgatttttc ttcttcagga gatggaaaca
      241 gtttttatac tcagtgggga aatcaagaaa ccagtaatag tggaagggga agagtaattc
      301 aagatgcaga agaaaggcca cattctcgat accttcgtag agcttattcc tctgatagat
      361 ctggcacttc taatagtcag tctcaagcaa aaacatatac aatggaacga tgtcactcag
      421 cagaaatgct ttcagtgtcc aaaagatcag gaggaggtga aaatgaagag aggtactcac
      481 ccacagacaa caatgccaac atttttaact tctttaaaga aaagacatcc agtagttctg
      541 gatcttttga aagacctgat aacaatcaag cactctccaa tcatctttgt ccaggaaaaa
      601 ctccttttcc atttgcagac ccgacacctc agactgaaac cgtacaacag tggtttggga
      661 atctgcaaat aaatgctcat ttaagaaaaa ctactgaata tgacagcatc agcccaaacc
      721 gggacttcca gggccatcca gatttgcaga aggacacatc
//
