LOCUS       sequence-13185-e        3486 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1328-HQ858829
FEATURES             Location/Qualifiers
     source          1..3486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1043..1142)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1159..1178)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1160..1178)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1183..1199)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1183..1199)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1301..1320)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1312..2121
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1379..1398)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2268..2856
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2757..2776
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3010..3027
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3186..3213)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3305..3391)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3440..3462
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3454..3471
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3455..3471
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atggctgctg ctgaggcggc ggcggcggtg gggaagcagc agcagaaggg cggcggggga
      181 aggggaggag gcggcggtgg gccggcgccg ttcctgacga agacgaacca gatggtggag
      241 gagagcgcga cggacgaggt gatctcgtgg ggaaaggagg ggcggtcgtt cgtggtgtgg
      301 aagccggtgg agttcgcccg cgacctcctc ccgctccact tcaagcactg caacttctcc
      361 tccttcgtcc gccagctcaa cacctacggt ttccggaagg tggtgcccga tcggtgggag
      421 ttcgccaacg gcaacttccg gcgaggagag caaggcctcc tctccggcat ccgccgccgc
      481 aaggcgacga cgcctcagtc ctccaaatcc tgcggcagcg gcgtcaacgt cgcgttccct
      541 ccgccgctgc cccctctgcc cccggagccg tcggcgacca cgtccagcgg caacgaccgc
      601 agctcctcct ccgcgtcctc gccgccgcgg gcggacatca ccagcgagaa cgagcagctc
      661 aggaaggaca accaaacgct gacgatggag ctggcgcggg ctcggcggca ctgcgaggag
      721 cttctgggct tcctgtcgcg cttcctcgac gtccggcagc tcgatctccg gctgctcatg
      781 caggaagaca tgcgagcggc ggccggcggc gtcggcggcg agcagcgcgt gcaagagcac
      841 gcccgggagg agaagtgcgt gaagctgttc ggcgtgctcc tcgacgacac ccatggcgcc
      901 gccacgagga agagggcgcg gtgcgaggag gcggctgcca gcgagcggcc gatcaagatg
      961 atcaggatcg gcgagccgtg ggtcagcgtt ccatcgtcgg ggccggcgcg gtgtggcggc
     1021 gacaacaagg gtgggcgcgc cgacccagct ttcttgtaca aagttggcat tataagaaag
     1081 cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
     1141 tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     1201 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     1261 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
     1321 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     1381 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg
     1441 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     1501 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     1561 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca
     1621 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     1681 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     1741 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     1801 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     1861 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     1921 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     1981 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     2041 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     2101 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
     2161 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg
     2221 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2281 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2341 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     2401 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     2461 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     2521 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     2581 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     2641 agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac
     2701 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     2761 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     2821 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     2881 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     2941 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     3001 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     3061 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     3121 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa
     3181 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
     3241 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
     3301 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     3361 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
     3421 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc
     3481 gggccc
//
