LOCUS       sequence-13186-e        4005 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1329-HQ858830
FEATURES             Location/Qualifiers
     source          1..4005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1562..1661)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1678..1697)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1679..1697)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1702..1718)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1702..1718)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1820..1839)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1831..2640
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1898..1917)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2787..3375
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3276..3295
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3529..3546
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3705..3732)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3824..3910)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3959..3981
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3973..3990
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3974..3990
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atggcgggga gtgggaggcc gccggcggcg cagaagaaga tcctgcagtc gctgcgcccg
      181 ccgctgccgt tcgccgcctc gtcgaggtcg cccttcgcgg cgcccaacga ctaccaccgc
      241 ttccccgctg gtggcgcggc ggcggcggcg gcgtctggtt cgggtggcat cggcgcgggc
      301 ggtgctggcg gcggcggcga tatcgaggag gggctggtca tccggacgcc gcaaaaaaga
      361 aaagcaccag aagaaagtga tgttgctgag tctagcgact gtatgattac tagccctgga
      421 tttgctgtca gtccaatgct cactccagtc tctgggaaag ctgttaagac ttccaagtca
      481 aagactaaga acaacaaagc tgggcctcag acacctacat caaatgttgg ttcaccactt
      541 aatcccccaa ctcctgttgg tacatgccgt tatgacagtt cgttagggct tctcacaaag
      601 aagttcataa atttgctgaa gcaggctccg gatggcattc tagatttgaa taatgctgca
      661 gaaacactag aggttcaaaa acggcgtata tatgatatta ccaatgtact tgaaggaata
      721 gggttgattg aaaagacact caagaacaga atccgttgga agggcttgga tgattcagga
      781 gtggaattag ataatggtct ttcagctttg caggcagaag ttgaaaatct tagtctgaag
      841 gagcaagcat tagatgagcg cataagtgat atgcgtgaaa aactaagagg tttaactgaa
      901 gatgaaaaca atcaaagatg gctctatgtg acagaagatg atatcaaggg attaccctgc
      961 tttcagaatg aaacactaat tgcaataaaa gctcctcatg gcactacact tgaagtccca
     1021 gatcctgacg aggctggtga ttatctccag agaagatata gaatcgtatt aagaagtaca
     1081 atgggcccaa tagatgtcta cttagttagt caatttgatg agaaatttga ggacttgggt
     1141 gggggtgcaa caccatcagg gcatgcaaat gtaccaaaac atcaacctac tgaagtcttc
     1201 aatacaacaa atgctggggt tgggcaatgt agcaactcag ttgctgtgga caataatatt
     1261 cagcacagcc agacgattcc tcaggatcct agtgcttcac atgattttgg aggaatgaca
     1321 aggattatcc cttcagatat tgatactgat gctgattact ggctcatatc agaaggggat
     1381 gtcagcatta ctgatatgtg gaaaacagca ccagatgtgc agtgggatga gagcctggat
     1441 acggatgtct tcctatctga agacgttaga acgccaagtt cacacaatca gcagccatct
     1501 gcagtgggcg ggccacagat gcaggtctca gacatgcata aacccaaggg tgggcgcgcc
     1561 gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc
     1621 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc
     1681 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
     1741 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
     1801 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
     1861 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     1921 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
     1981 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     2041 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     2101 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa
     2161 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     2221 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     2281 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     2341 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     2401 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     2461 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     2521 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     2581 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     2641 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     2701 gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     2761 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2821 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2881 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     2941 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     3001 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     3061 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3121 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat
     3181 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3241 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3301 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     3361 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3421 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     3481 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     3541 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     3601 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     3661 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     3721 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     3781 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     3841 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc
     3901 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc
     3961 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccc
//
