LOCUS       sequence-131879-        1083 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a 3xFLAG-tagged TAL protein recognizing a telomere 
            repeat..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Fujita T, Asano Y, Ohtsuka J, Takada Y, Saito K, Ohki R, Fujii H
  TITLE     Identification of telomere-associated molecules by engineered 
            DNA-binding molecule-mediated chromatin immunoprecipitation 
            (enChIP).
  JOURNAL   Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038/srep03171.
  PUBMED    24201379
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-11-8"; volume: "3:3171. doi"; pages: " 
            10.1038/srep03171"
COMMENT     Sequence Label: 3xFN-Tel-TAL/pCMV-7.1
FEATURES             Location/Qualifiers
     source          1..1083
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..111
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      127..136
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             136..177
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             190..210
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagaattaa ccatggacta caaagaccat
       61 gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa gcttgcggcc
      121 ggccgcgcca ccatgggaaa acctattcct aatcctctgc tgggcctgga ttctaccgga
      181 ggcatggccc ctaagaaaaa gcggaaggtg gacggcggag tggacctgag aacactggga
      241 tattctcagc agcagcagga gaagatcaag cccaaggtga gatccacagt ggcccagcac
      301 cacgaagccc tggtgggaca cggatttaca cacgcccaca ttgtggccct gtctcagcac
      361 cctgccgccc tgggaacagt ggccgtgaaa tatcaggata tgattgccgc cctgcctgag
      421 gccacacacg aagccattgt gggagtggga aaacagtggt ctggagccag agccctggaa
      481 gccctgctga cagtggccgg agaactgaga ggacctcctc tgcagctgga tacaggacag
      541 ctgctgaaga ttgccaaaag gggcggagtg accgcggtgg aagccgtgca cgcctggaga
      601 aatgccctga caggagcccc tctgaacctg acccccgaac aggtggtggc cattgccagc
      661 aacatcggcg gcaagcaggc cctggaaacc gtgcagagac tgctgcccgt gctgtgccag
      721 gcccatggcc tgacacctga acaggtggtg gctatcgcct ctaacaacgg aggaaaacag
      781 gctctggaaa cagtgcagcg gctgctgcct gtgctgtgtc aggctcacgg cttgactcca
      841 gaacaggtgg tggctattgc ttccaacaac ggggggaaac aggccctgga aactgtgcag
      901 cgcctgctgc cagtgctgtg ccaggctcac ggactgaccc ccgaacaggt ggtggccatt
      961 gccagcaaca acggcggcaa gcaggccctg gaaaccgtgc agagactgct gcccgtgctg
     1021 tgccaggccc atggcctgac acctgaacag gtggtggcta tcgcctctaa cggcggagga
     1081 aaa
//
