LOCUS       sequence-13188-e        3558 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1331-HQ858832
FEATURES             Location/Qualifiers
     source          1..3558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1115..1214)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1231..1250)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1232..1250)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1255..1271)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1255..1271)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1373..1392)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1384..2193
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1451..1470)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2340..2928
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2829..2848
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3082..3099
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3258..3285)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3377..3463)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3512..3534
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3526..3543
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3527..3543
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atgattacca tggatctgat gggtgggtac gggcgggtgg acgagcaggt ggccatccag
      181 gaggcggcgg cggcggggct aagggggatg gagcatctta ttctgcagct gtcccagact
      241 gggacgagcg agaggtcgcc ggcgccggcg caggagcagc agcaacaggt ggactgcagg
      301 gagatcacgg acatgacggt gtccaagttc aagaaggtga tctccatgct gaaccgcacc
      361 ggccacgcgc ggttccggcg gggcccggtg gtggcgcagt cgtcgggccc ggcggcgtcc
      421 gagccggcgc cggtgaggtc gtccccgtcg gcggtgtcga ggcccatgac gctcgacttc
      481 accaaggcgg cgtccgggta cggcaaggac gccgggttca gcgtctccgg catctccgcc
      541 gcgagctcgt ccttcctctc gtcggtcacc ggcgacggca gcgtgtccaa cgggcgcggc
      601 ggcgggtcat cctccctgat gcttcccccg ccgccggcga ccagctgcgg caagccaccg
      661 ctgtcctccg ccgccgccgc catgtcagcc ggcgcaggcc acaagcgcaa gtgccacgac
      721 cacgcgcact ccgagaacgt cgccggcggc aagtacggat ccaccggcgg ccgctgccac
      781 tgctccaagc gccggaagca tcgggtgaag aggacgatcc gcgtgccggc gataagctcg
      841 aaggtggcgg acatccccgc cgacgacttc tcgtggcgga agtacgggca gaagcccatc
      901 aagggctccc ccttcccacg aggatactac aagtgtagca cgctgcgcgg ttgcccggcg
      961 aggaagcacg tggagcgcga cccgaccgac ccgtccatgc tcatcgtcac ctacgagggc
     1021 gagcaccgcc actccccctc cgccgccggc caggaccacc cgccggcgcc gcctccgccg
     1081 ctggcgctgc cgctcgccaa gggtgggcgc gccgacccag ctttcttgta caaagttggc
     1141 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
     1201 aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata
     1261 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     1321 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     1381 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
     1441 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     1501 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     1561 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     1621 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     1681 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     1741 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     1801 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     1861 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     1921 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     1981 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     2041 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     2101 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     2161 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     2221 tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac
     2281 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2341 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2401 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2461 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     2521 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2581 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2641 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2701 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     2761 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2821 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2881 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2941 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3001 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3061 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3121 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3181 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3241 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3301 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     3361 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     3421 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     3481 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     3541 agtcttaagc tcgggccc
//
