LOCUS       sequence-131889-        6296 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  luciferase reporter with 3 copies of the GM420 NFAT-AP-1 site in 
            front of hGM-CSF -55 to +28 minimal promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6296)
  AUTHORS   Johnson BV, Bert AG, Ryan GR, Condina A, Cockerill PN
  TITLE     Granulocyte-macrophage colony-stimulating factor enhancer activation
            requires cooperation between NFAT and AP-1 elements and is 
            associated with extensive nucleosome reorganization.
  JOURNAL   Mol Cell Biol. 2004 Sep;24(18):7914-30.
  PUBMED    15340054
REFERENCE   2  (bases 1 to 6296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2004-09"; volume: "24(18)"; pages: "7914-30"
COMMENT     Sequence Label: p420-3
FEATURES             Location/Qualifiers
     source          1..6296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      279..288
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             285..1937
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(351..369)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          2022..2087
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2217..2237
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2659..2793
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3564..3582)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3650..3754
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3755..3823,3824..4615)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3973..3992)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4786..5374
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5275..5294
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5528..5545
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5560..5700
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5626..5645)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5786..5808
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     polyA_signal    5939..6073
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    join(6167..6296,1..5)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ggatccaagc tcagatcccc tgatgtcatc tttccatgag aaagatggga tcccctgatg
       61 tcatctttcc atgagaaaga tgggatcccc tgatgtcatc tttccatgag aaagatggga
      121 tctgggcccg gatcctgcag ggggtcgaca agcttcgcga ggtcaccatt aatcatttcc
      181 tctgtgtatt taagagctct tttgccagtg agcccagtac acagagagaa aggctaaagt
      241 tctgtcgaca agcttggcat tccggtactg ttggtaaagc caccatggaa gacgccaaaa
      301 acataaagaa aggcccggcg ccattctatc cgctggaaga tggaaccgct ggagagcaac
      361 tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac
      421 atatcgaggt ggacatcact tacgctgagt acttcgaaat gtccgttcgg ttggcagaag
      481 ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc
      541 ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga
      601 acgacattta taatgaacgt gaattgctca acagtatggg catttcgcag cctaccgtgg
      661 tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca
      721 tccaaaaaat tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca
      781 cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgtg ccagagtcct
      841 tcgataggga caagacaatt gcactgatca tgaactcctc tggatctact ggtctgccta
      901 aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt ctcgcatgcc agagatccta
      961 tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg
     1021 gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt
     1081 atagatttga agaagagctg tttctgagga gccttcagga ttacaagatt caaagtgcgc
     1141 tgctggtgcc aaccctattc tccttcttcg ccaaaagcac tctgattgac aaatacgatt
     1201 tatctaattt acacgaaatt gcttctggtg gcgctcccct ctctaaggaa gtcggggaag
     1261 cggttgccaa gaggttccat ctgccaggta tcaggcaagg atatgggctc actgagacta
     1321 catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg
     1381 ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc
     1441 aaagaggcga actgtgtgtg agaggtccta tgattatgtc cggttatgta aacaatccgg
     1501 aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact
     1561 gggacgaaga cgaacacttc ttcatcgttg accgcctgaa gtctctgatt aagtacaaag
     1621 gctatcaggt ggctcccgct gaattggaat ccatcttgct ccaacacccc aacatcttcg
     1681 acgcaggtgt cgcaggtctt cccgacgatg acgccggtga acttcccgcc gccgttgttg
     1741 ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag
     1801 taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc
     1861 ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg
     1921 gaaagatcgc cgtgtaattc tagaggatct ttgtgaagga accttacttc tgtggtgtga
     1981 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
     2041 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
     2101 gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag
     2161 aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa
     2221 aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga
     2281 gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa
     2341 aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc
     2401 ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg
     2461 ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta
     2521 ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
     2581 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     2641 atgaatgcaa ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     2701 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     2761 aaactcatca atgtatctta tcatgtctgg atcgatcccc aggaagctcc tctgtgtcct
     2821 cataaaccct aacctcctct acttgagagg acattccaat cataggctgc ccatccaccc
     2881 tctgtgtcct cctgttaatt aggtcactta acaaaaagga aattgggtag gggtttttca
     2941 cagaccgctt tctaagggta attttaaaat atctgggaag tcccttccac tgctgtgttc
     3001 cagaagtgtt ggtaaacagc ccacaaatgt caacagcaga aacatacaag ctgtcagctt
     3061 tgcacaaggg cccaacaccc tgctcatcaa gaagcactgt ggttgctgtg ttagtaatgt
     3121 gcaaaacagg aggcacattt tccccacctg tgtaggttcc aaaatatcta gtgttttcat
     3181 ttttacttgg atcaggaacc cagcactcca ctggataagc attatcctta tccaaaacag
     3241 ccttgtggtc agtgttcatc tgctgactgt caactgtagc attttttggg gttacagttt
     3301 gagcaggata tttggtcctg tagtttgcta acacaccctg cagctccaaa ggttccccac
     3361 caacagcaaa aaaatgaaaa tttgaccctt gaatgggttt tccagcacca ttttcatgag
     3421 ttttttgtgt ccctgaatgc aagtttaaca tagcagttac cccaataacc tcagttttaa
     3481 cagtaacagc ttcccacatc aaaatatttc cacaggttaa gtcctcattt aaattaggca
     3541 aaggaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     3601 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     3661 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     3721 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     3781 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     3841 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     3901 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     3961 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
     4021 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     4081 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     4141 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     4201 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     4261 agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg
     4321 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     4381 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     4441 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     4501 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     4561 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     4621 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     4681 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     4741 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     4801 tctgcgcgta atatgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     4861 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     4921 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     4981 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5041 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     5101 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     5161 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     5221 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     5281 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     5341 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     5401 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     5461 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     5521 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     5581 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     5641 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     5701 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     5761 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     5821 tcaccgaaac gcgcgaggca ggatcagcca tatcacattt gtagaggttt tacttgcttt
     5881 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttaa
     5941 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     6001 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     6061 tcatgtctgg atcagccata tcacatttgt agaggtttta cttgctttaa aaaacctccc
     6121 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttaact tgtttattgc
     6181 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     6241 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtct
//
