LOCUS       sequence-13189-e        4359 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1332-HQ858833
FEATURES             Location/Qualifiers
     source          1..4359
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1916..2015)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2032..2051)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2033..2051)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2056..2072)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2056..2072)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2174..2193)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2185..2994
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2252..2271)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3141..3729
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3630..3649
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3883..3900
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4059..4086)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4178..4264)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4313..4335
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4327..4344
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4328..4344
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atgatgggag cagcggtgac catggtggat cggcgcatgg cgttcgcggc ggaggccgac
      181 gttgatagca aggccgcctt tggattcttc ggaggcgagt gcttcgtcgg cgagggcgac
      241 ctggtcaacc cggcgccgcc gccgccgcag cagcagcagg tgcacgaggg cggattcgcc
      301 gccgaggacg agagcgacgg cgacgacgac gacgacgacg acgacgacgt ggacgacatc
      361 gaggagctgg agcgccgcat gtggcgcgac cgcgtccggc acaagcggct caaggagctt
      421 cagcagagcc gcgccgggag ggagagccgg gcgggcgacg ccggcggcgg aggcaggcag
      481 cagcggcagt cgcaggagca ggcgcggcgg aagaagatgt cgcgggcgca ggacgggatc
      541 ctcaagtaca tgctcaagat gatggaggtg tgcaacgcgc aggggttcgt gtacggcatc
      601 atcccggaga aggggaagcc ggtgagcggc gcctccgaca acctccgctc atggtggaag
      661 gagaaggtcc gcttcgaccg caatggcccg gccgccatcg ccaagtacca ggccgacaac
      721 gccgtgccgg ggtgcgacgg cgacgccggc ggcgccgcgc cggcggggcc gcactccctg
      781 cacgagctgc aggacaccac gctgggatcg ctgctctcgg cgctcatgca gcactgcgac
      841 ccgccgcagc gccgcttccc gctggagaag ggcgtgccgc cgccgtggtg gccggagggg
      901 agcgaggcgt ggtggcccga ggccggcgtg cccaaggagc tcggcccgcc gccgtacaag
      961 aagccgcacg acctcaagaa ggcgtggaag gtcgccgtgc tcaccgccgt catcaagcac
     1021 atgtcccccg acgtcgacaa ggtccgccgc ctcgtgcgtc agtccaagtg cctccaggac
     1081 aagatgaccg ccaaggagat cgtcacctgg ctcgccgtcc tcaagcagga ggaggacctc
     1141 tatctcaagc tccatcccgg cgccctcccc ccgccgctct ccgccgcgtc cttcaacgcc
     1201 agcgtctccg gcgagtacga cgtcgaagga gtcgacggcg acgaggccgg gaacaacaac
     1261 ctgcagaagg cgcagaacga cgcgaccgcg ttcatggacc tcaccaccac catggatgct
     1321 gctctgagca acaacaagtt cctgatcatg ccgctgatga aggaagaggc catcgacgtc
     1381 gacttcatcc agaagaggag cgagccggag ctgatgctga gcagcgacag ccatgcccgc
     1441 gtctacacct gcggcaacgt gcagtgcccg cacagcaact acgcgctcgg cttcctcgac
     1501 cgcaacgaac gcaacgccca tcagtacgcc tgcaagcaca acgccgccgc cgccgccgcc
     1561 gagagcaagc cgccgccgcc gcacatcttc gagccactcg gcagcttcga cttcgacctc
     1621 cccgtcgacg gccagagatg cctcgccggg ctgatgacca tgtacgacaa cgacgtcgcc
     1681 gccgccacgc agatgcacca ccaccaccat cagcagcagc aggctaattt cttcatccgc
     1741 gacgacgcgc cgttcggagg cgacgtcgcc gccacggcgg cggcggcgcc ggagttcagg
     1801 ttcagttcaa atttcaacgt gacaggaggg ggcgccgtgg actacggcgg cgccatgcag
     1861 caaccgccgg cgaagtacgc cggatccaac tggttctaca agggtgggcg cgccgaccca
     1921 gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
     1981 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt
     2041 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     2101 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     2161 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
     2221 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     2281 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
     2341 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     2401 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     2461 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
     2521 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     2581 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     2641 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     2701 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     2761 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     2821 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     2881 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     2941 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     3001 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     3061 tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     3121 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3181 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3241 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     3301 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3361 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3421 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3481 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa
     3541 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3601 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3661 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3721 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3781 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3841 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     3901 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     3961 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     4021 acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
     4081 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
     4141 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
     4201 tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta
     4261 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca
     4321 cgacgttgta aaacgacggc cagtcttaag ctcgggccc
//
