LOCUS       sequence-131891-        6766 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  luciferase reporter with human GM-CSF promoter  -627 to + 28.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6766)
  AUTHORS   Cockerill PN, Bert AG, Roberts D, Vadas MA
  TITLE     The human granulocyte-macrophage colony-stimulating factor gene is 
            autonomously regulated in vivo by an inducible tissue-specific 
            enhancer.
  JOURNAL   Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):15097-102.
  PUBMED    10611344
REFERENCE   2  (bases 1 to 6766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1999-12-21"; volume: "96(26)"; pages: 
            "15097-102"
COMMENT     Sequence Label: pGM
FEATURES             Location/Qualifiers
     source          1..6766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      749..758
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             755..2407
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(821..839)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          2492..2557
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2687..2707
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3129..3263
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4034..4052)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4120..4224
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4225..4293,4294..5085)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4443..4462)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5256..5844
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5745..5764
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5998..6015
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6030..6170
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6096..6115)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6256..6278
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     polyA_signal    6409..6543
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    join(6637..6766,1..5)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ggatccaagc tcagatctcg agctcggtac ccgggtcgac aagcttcgcg aggtcaccag
       61 cttgctgaga gtggctgcag tctcgctgct ggatgtgcac atggtggtca ttccctctgc
      121 tcacaggggc aggggtcccc ccttactgga ctgaggttgc cccctgctcc aggtcctggg
      181 tgggagccca tgtgaactgt cagtggggca ggtctgtgag agctcccctc acactcaagt
      241 ctctcacagt ggccagagaa gaggaaggct ggagtcagaa tgaggcacca gggcgggcat
      301 agcctgccca aaggcccctg ggattacagg caggatgggg agccctatct aagtgtctcc
      361 cacgccccac cccagccatt ccaggccagg aagtccaaac tgtgcccctc agagggaggg
      421 ggcagcctca ggcccattca gactgcccag ggagggctgg agagccctca ggaaggcgag
      481 tgggtgggct gtcggttctt ggaaaggttc attaatgaaa acccccaagc ctgaccacct
      541 agggaaaagg ctcaccgttc ccatgtgtgg ctgataaggg ccaggagatt ccacagttca
      601 ggtagttccc ccgcctccct ggcattttgt ggtcaccatt aatcatttcc tctgtgtatt
      661 taagagctct tttgccagtg agcccagtac acagagagaa aggctaaagt tctgtcgaca
      721 agcttggcat tccggtactg ttggtaaagc caccatggaa gacgccaaaa acataaagaa
      781 aggcccggcg ccattctatc cgctggaaga tggaaccgct ggagagcaac tgcataaggc
      841 tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac atatcgaggt
      901 ggacatcact tacgctgagt acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg
      961 atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc ttcaattctt
     1021 tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga acgacattta
     1081 taatgaacgt gaattgctca acagtatggg catttcgcag cctaccgtgg tgttcgtttc
     1141 caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca tccaaaaaat
     1201 tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca cgttcgtcac
     1261 atctcatcta cctcccggtt ttaatgaata cgattttgtg ccagagtcct tcgataggga
     1321 caagacaatt gcactgatca tgaactcctc tggatctact ggtctgccta aaggtgtcgc
     1381 tctgcctcat agaactgcct gcgtgagatt ctcgcatgcc agagatccta tttttggcaa
     1441 tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg gttttggaat
     1501 gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt atagatttga
     1561 agaagagctg tttctgagga gccttcagga ttacaagatt caaagtgcgc tgctggtgcc
     1621 aaccctattc tccttcttcg ccaaaagcac tctgattgac aaatacgatt tatctaattt
     1681 acacgaaatt gcttctggtg gcgctcccct ctctaaggaa gtcggggaag cggttgccaa
     1741 gaggttccat ctgccaggta tcaggcaagg atatgggctc actgagacta catcagctat
     1801 tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg ttccattttt
     1861 tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc aaagaggcga
     1921 actgtgtgtg agaggtccta tgattatgtc cggttatgta aacaatccgg aagcgaccaa
     1981 cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact gggacgaaga
     2041 cgaacacttc ttcatcgttg accgcctgaa gtctctgatt aagtacaaag gctatcaggt
     2101 ggctcccgct gaattggaat ccatcttgct ccaacacccc aacatcttcg acgcaggtgt
     2161 cgcaggtctt cccgacgatg acgccggtga acttcccgcc gccgttgttg ttttggagca
     2221 cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag taacaaccgc
     2281 gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa
     2341 actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg gaaagatcgc
     2401 cgtgtaattc tagaggatct ttgtgaagga accttacttc tgtggtgtga cataattgga
     2461 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg
     2521 tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga
     2581 atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc
     2641 atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag
     2701 aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt
     2761 gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact
     2821 gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta
     2881 taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa
     2941 ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata
     3001 tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt
     3061 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     3121 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     3181 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     3241 atgtatctta tcatgtctgg atcgatcccc aggaagctcc tctgtgtcct cataaaccct
     3301 aacctcctct acttgagagg acattccaat cataggctgc ccatccaccc tctgtgtcct
     3361 cctgttaatt aggtcactta acaaaaagga aattgggtag gggtttttca cagaccgctt
     3421 tctaagggta attttaaaat atctgggaag tcccttccac tgctgtgttc cagaagtgtt
     3481 ggtaaacagc ccacaaatgt caacagcaga aacatacaag ctgtcagctt tgcacaaggg
     3541 cccaacaccc tgctcatcaa gaagcactgt ggttgctgtg ttagtaatgt gcaaaacagg
     3601 aggcacattt tccccacctg tgtaggttcc aaaatatcta gtgttttcat ttttacttgg
     3661 atcaggaacc cagcactcca ctggataagc attatcctta tccaaaacag ccttgtggtc
     3721 agtgttcatc tgctgactgt caactgtagc attttttggg gttacagttt gagcaggata
     3781 tttggtcctg tagtttgcta acacaccctg cagctccaaa ggttccccac caacagcaaa
     3841 aaaatgaaaa tttgaccctt gaatgggttt tccagcacca ttttcatgag ttttttgtgt
     3901 ccctgaatgc aagtttaaca tagcagttac cccaataacc tcagttttaa cagtaacagc
     3961 ttcccacatc aaaatatttc cacaggttaa gtcctcattt aaattaggca aaggaattct
     4021 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     4081 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4141 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     4201 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     4261 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     4321 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     4381 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     4441 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     4501 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     4561 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     4621 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     4681 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     4741 aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta
     4801 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     4861 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     4921 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     4981 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     5041 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     5101 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5161 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5221 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5281 atatgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5341 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5401 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     5461 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     5521 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     5581 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     5641 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     5701 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     5761 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     5821 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     5881 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     5941 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     6001 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
     6061 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
     6121 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
     6181 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     6241 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     6301 gcgcgaggca ggatcagcca tatcacattt gtagaggttt tacttgcttt aaaaaacctc
     6361 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttaa cttgtttatt
     6421 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6481 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     6541 atcagccata tcacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     6601 ctgaacctga aacataaaat gaatgcaatt gttgttaact tgtttattgc agcttataat
     6661 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     6721 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtct
//
