LOCUS       sequence-131894-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expressed human optimized S. pyogenese dCas9 fused to KRAB 
            repressor domain and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Kabadi AM, Ousterout DG, Hilton IB, Gersbach CA
  TITLE     Multiplex CRISPR/Cas9-based genome engineering from a single 
            lentiviral vector.
  JOURNAL   Nucleic Acids Res. 2014 Aug 13. pii: gku749.
  PUBMED    25122746
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Aug 13. pii: gku749."
COMMENT     Sequence Label: pLV hUbC-dCas9 KRAB-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      132..141
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             141..206
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             213..233
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt agactagtaa
       61 attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagctt gggctgcagg
      121 tcgactctag agccaccatg gactacaaag accatgacgg tgattataaa gatcatgaca
      181 tcgattacaa ggatgacgat gacaagatgg cccccaagaa gaagaggaag gtgggccgcg
      241 gaatggacaa gaagtactcc attgggctcg ccatcggcac aaacagcgtc ggctgggccg
      301 tcattacgga cgagtacaag gtgccgagca aaaaattcaa agttctgggc aataccgatc
      361 gccacagcat aaagaagaac ctcattggcg ccctcctgtt cgactccggg gaaaccgccg
      421 aagccacgcg gctcaaaaga acagcacggc gcagatatac ccgcagaaag aatcggatct
      481 gctacctgca ggagatcttt agtaatgaga tggctaaggt ggatgactct ttcttccata
      541 ggctggagga gtcctttttg gtggaggagg ataaaaagca cgagcgccac ccaatctttg
      601 gcaatatcgt ggacgaggtg gcgtaccatg aaaagtaccc aaccatatat catctgagga
      661 agaagcttgt agacagtact gataaggctg acttgcggtt gatctatctc gcgctggcgc
      721 atatgatcaa atttcgggga cacttcctca tcgaggggga cctgaaccca gacaacagcg
      781 atgtcgac
//
