LOCUS       sequence-131899-         393 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for SaCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Kleinstiver BP, Prew MS, Tsai SQ, Nguyen NT, Topkar VV, Zheng Z, 
            Joung JK
  TITLE     Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 
            by modifying PAM recognition.
  JOURNAL   Nat Biotechnol. 2015 Nov 2. doi: 10.1038/nbt.3404.
  PUBMED    26524662
REFERENCE   2  (bases 1 to 393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Nov 2. doi: 10.1038/nbt.3404."
COMMENT     Sequence Label: MSP2283
FEATURES             Location/Qualifiers
     source          1..393
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     110..129
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        110..128
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    129..153
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg
       61 tgtccgggat ctcgacgctc tcccttatgc gactcctgca ttaggaaatt aatacgactc
      121 actatagggg aattgtgagc ggataacaat tcccctgtag aaataatttt gtttaacttt
      181 aataaggaga tataccatgg gcaaacggaa ctacatcctg gggcttgaca ttgggataac
      241 cagcgttggc tacggaatta ttgattatga gacacgcgat gtgattgacg ccggggttag
      301 gctgttcaaa gaggccaacg ttgaaaacaa cgagggaaga cggagtaagc gcggagcaag
      361 aagactcaag cgcagacgga gacatcggat tca
//
