LOCUS       sequence-13190-e        4434 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1333-HQ858834
FEATURES             Location/Qualifiers
     source          1..4434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1991..2090)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2107..2126)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2108..2126)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2131..2147)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2131..2147)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2249..2268)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2260..3069
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2327..2346)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3216..3804
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3705..3724
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3958..3975
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4134..4161)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4253..4339)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4388..4410
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4402..4419
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4403..4419
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atggccgatt cgccaaaccc tagctccggt gaccaccccg cgggcgtcgg cgggtcgccg
      181 gagaagcagc ccccggtgga tcggcgcgtc gcggcgctcg ccgcgggcgc ggcgggcgcg
      241 ggggcgaggt acaaggcgat gtcccccgcg cggctgccga tctcgcggga gccctgcctc
      301 accatccccg cgggcttcag cccctcggct ctcctcgagt cccccgtcct cctcaccaac
      361 ttcaaggttg aaccctctcc gacaactggt actctgagca tggctgcaat tatgaacaag
      421 agtgcaaatc cagacatact tccttcgcct agggataaaa catctggtag cacccatgaa
      481 gatggtggct ctcgagattt tgaattcaag cctcatctga attcatcctc tcaatcgacg
      541 gcttctgcta tcaatgatcc caaaaagcat gaaacttcta tgaaaaatga aagcctgaat
      601 actgccctgt catctgacga tatgatgatc gacaatatac ctctatgttc tcgtgagtca
      661 actctcgcag tcaatatttc aagtgccccg agccaactgg ttggaatggt tggtttaact
      721 gacagctcac ctgctgaagt tggtacatct gagttgcatc agatgaatag ctctggaaat
      781 gctatgcagg agtcacagcc tgaaagtgtg gctgaaaagt ctgcagagga tggttataac
      841 tggcgcaaat atgggcaaaa gcatgttaag ggaagtgaga acccgagaag ctattacaag
      901 tgcacacatc ctaactgtga tgtaaaaaag ctattggagc gttcgcttga tggtcagatt
      961 actgaagtgg tttataaagg gcgtcacaat caccctaagc cccaacccaa taggaggctg
     1021 tctgccggtg cagttcctcc aatccagggt gaagaaagat atgatggtgt ggcaactact
     1081 gatgacaaat cttcaaatgt tcttagcatt cttggtaatg cagtacatac agctggtatg
     1141 attgagcctg ttccaggctc agctagtgat gatgacaatg atgccggagg agggagacct
     1201 taccctggag atgatgctgt tgaggatgat gatttagagt caaaacgaag gaaaatggaa
     1261 tctgctgcta ttgatgctgc tttgatgggc aagcctaacc gtgagcctcg tgttgtagta
     1321 caaacggtta gtgaggttga catcttggat gatgggtacc gctggcgcaa gtatggccag
     1381 aaagtagtta aaggaaaccc caatccacgg agttactaca agtgcacaaa tacaggatgc
     1441 ccagtcagga agcatgttga gagagcatca catgatccaa aatcagtcat aacaacatat
     1501 gaaggaaaac ataaccatga agtccctgcg tcgaggaatg cgagccatga gatgtccact
     1561 ccccccatga agcctgttgt ccatccaatt aacagcaata tgcagggcct tggtggcatg
     1621 atgagagcat gtgaacctag gacatttcca aaccaatatt ctcaagcagc tgaaagtgac
     1681 accatcagcc ttgatcttgg tgttggaatc agcccgaacc acagcgatgc cacaaaccaa
     1741 ttgcagtcct cagtttctga tcagatgcag tatcaaatgc agcccatggg ttcagtatac
     1801 agtaatatgg gacttccagc aatggcaatg ccgactatgg ctggcaatgc agctagcaat
     1861 atatatggtt cgagagaaga aaaacctagt gaaggtttta ctttcaaagc cacaccgatg
     1921 gaccattcgg ctaacttatg ctacagtacc gccggcaatt tagtcatggg tccgaagggt
     1981 gggcgcgccg acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
     2041 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
     2101 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     2161 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
     2221 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
     2281 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     2341 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
     2401 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     2461 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     2521 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta
     2581 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     2641 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     2701 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     2761 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
     2821 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     2881 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     2941 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     3001 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     3061 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     3121 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga
     3181 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     3241 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     3301 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     3361 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     3421 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     3481 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3541 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3601 cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3661 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3721 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3781 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3841 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3901 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3961 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     4021 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     4081 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
     4141 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
     4201 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
     4261 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
     4321 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
     4381 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccc
//
