LOCUS       sequence-131904-         300 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for Sa-dCas9 (D10A/H557A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Kleinstiver BP, Prew MS, Tsai SQ, Nguyen NT, Topkar VV, Zheng Z, 
            Joung JK
  TITLE     Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 
            by modifying PAM recognition.
  JOURNAL   Nat Biotechnol. 2015 Nov 2. doi: 10.1038/nbt.3404.
  PUBMED    26524662
REFERENCE   2  (bases 1 to 300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Nov 2. doi: 10.1038/nbt.3404."
COMMENT     Sequence Label: MSP2262
FEATURES             Location/Qualifiers
     source          1..300
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    71..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggtgtccggg atctcgacgc tctcccttat gcgactcctg cattaggaaa ttaatacgac
       61 tcactatagg ggaattgtga gcggataaca attcccctgt agaaataatt ttgtttaact
      121 ttaataagga gatataccat gggcaaacgg aactacatcc tggggcttgc cattgggata
      181 accagcgttg gctacggaat tattgattat gagacacgcg atgtgattga cgccggggtt
      241 aggctgttca aagaggccaa cgttgaaaac aacgagggaa gacggagtaa gcgcggagca
//
