LOCUS       sequence-131907-         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for SaCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Kleinstiver BP, Prew MS, Tsai SQ, Nguyen NT, Topkar VV, Zheng Z, 
            Joung JK
  TITLE     Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 
            by modifying PAM recognition.
  JOURNAL   Nat Biotechnol. 2015 Nov 2. doi: 10.1038/nbt.3404.
  PUBMED    26524662
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Nov 2. doi: 10.1038/nbt.3404."
COMMENT     Sequence Label: MSP2266
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(251..298)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     294..312
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     promoter        complement(424..442)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(512..577)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(584..604)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tactgcgatg agtggcaggg cggggcgtaa tttttttaag gcagttattg gtgcccttaa
       61 acgcctggtt gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgaaa
      121 gcaaattcga cccggtcgtc ggttcagggc agggtcgtta aatagccgct tatgtctatt
      181 gctggtttac cggtttattg actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct
      241 gaggtttcag caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt tatgctagtt
      301 attgctcagc ggaaaaaaaa tctcgccaac aagttgacga gataaacacg gcattttgcc
      361 ttgttttagt agattctgta attttcatta cagagtacta aaacaggtga agttcgaggg
      421 cgacctatag tgagtcgtat taatttcgat tatgcggccg tgtacaatac gattactttc
      481 tgttcgactt aagcattatg cggccgcttt acttgtcatc gtcatccttg taatcgatgt
      541 catgatcttt ataatcaccg tcatggtctt tgtagtcgct cgagactttc ctcttcttct
      601 tgggggatcc acctttttta atgatttgtg ggtgcttctt tgacttcact tcatagagat
      661 tccccaggat atcggtagag tactttttta ttgactgagt tttagatgcg attgtcttga
      721 tgatgcgagg cggccgttta tcattcatgt tttcgagata ctccc
//
