LOCUS       sequence-131913-         602 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for KKH SaCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Kleinstiver BP, Prew MS, Tsai SQ, Nguyen NT, Topkar VV, Zheng Z, 
            Joung JK
  TITLE     Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 
            by modifying PAM recognition.
  JOURNAL   Nat Biotechnol. 2015 Nov 2. doi: 10.1038/nbt.3404.
  PUBMED    26524662
REFERENCE   2  (bases 1 to 602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Nov 2. doi: 10.1038/nbt.3404."
COMMENT     Sequence Label: MSP2292
FEATURES             Location/Qualifiers
     source          1..602
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     116..135
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        116..134
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    135..159
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt
       61 cgatggtgtc cgggatctcg acgctctccc ttatgcgact cctgcattag gaaattaata
      121 cgactcacta taggggaatt gtgagcggat aacaattccc ctgtagaaat aattttgttt
      181 aactttaata aggagatata ccatgggcaa acggaactac atcctggggc ttgacattgg
      241 gataaccagc gttggctacg gaattattga ttatgagaca cgcgatgtga ttgacgccgg
      301 ggttaggctg ttcaaagagg ccaacgttga aaacaacgag ggaagacgga gtaagcgcgg
      361 agcangaaga ctcaagcgca gacggagaca tcggattcag agggtgaaaa agctgctctt
      421 cgattacaat ctcctgaccg atcatagtga gctgagcgga atcaacccct acgaggcgcg
      481 agtgaaaggg ctttcccaga agctgtccga agaggagttc tccgccgcgt tgctgcacct
      541 ggccaaacgg aggggggttc acaatgtaaa cgaagtggag gaggacacgg gcaatgaact
      601 ta
//
