LOCUS       sequence-131917-         602 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RSF1010-based vector with extensive polylinker, and resistance to 
            Strep and Spec. Shuttle vector suitable for transfer to, and 
            replication in, E. coli and Anabaena (Nostoc) sp. strain PCC 7120..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Wolk CP, Fan Q, Zhou R, Huang G, Lechno-Yossef S, Kuritz T, Wojciuch
            E
  TITLE     Paired cloning vectors for complementation of mutations in the 
            cyanobacterium Anabaena sp. strain PCC 7120.
  JOURNAL   Arch Microbiol. 2007 Dec;188(6):551-63. Epub 2007 Jul 17.
  PUBMED    17639350
REFERENCE   2  (bases 1 to 602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Microbiol."; date: "2007-12"; volume: "188(6)"; pages: "551-63. Epub
            2007 Jul 17"
COMMENT     Sequence Label: pRL1383a
FEATURES             Location/Qualifiers
     source          1..602
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(120..137)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(312..331)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tcgaattcat cgatctcgta acccgggccc gcggatgcat atgatcagat cttaaggcct
       61 aggtctagac gtcgtacgtg cactcgagcc cgggcgcgcc ggaccggccg gcagggatcc
      121 tatagtgtca cctaaatcgt atgcggccgc tcgtaagagg ttccaacttt caccataatg
      181 aaataagatc actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag
      241 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
      301 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcaga
      361 aaataaaaaa ggggacctct agggtcccca attaattagt aatataatct attaaaggtc
      421 attcaaaagg tcatccaccg gatcaattcc cctgctcgcg caggctgggt gccaagctct
      481 cgggtaacat caaggcccga tccttggagc ccttgccctc ccgcacgatg atcgtgccgt
      541 gatcgaaatc cagatccttg acccgcagtt gcaaaccctc actgatccgc ccgttccata
      601 ca
//
