LOCUS       sequence-131919-         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector suitable for transfer to Anabaena (Nostoc) sp. strain
            PCC 7120 and other cyanobacterial strains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Wolk CP, Cai Y, Cardemil L, Flores E, Hohn B, Murry M, Schmetterer 
            G, Schrautemeier B, Wilson R
  TITLE     Isolation and complementation of mutants of Anabaena sp. strain PCC 
            7120 unable to grow aerobically on dinitrogen.
  JOURNAL   J Bacteriol. 1988 Mar;170(3):1239-44.
  PUBMED    2830231
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol."; date: "1988-03"; volume: "170(3)"; pages: "1239-44"
COMMENT     Sequence Label: pRL25
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(408..413,414..430,431..436))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
       61 ttttggtcat gagattatca aaaaggatcc ttttaaatta aaaatgaagt tttaaatcaa
      121 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
      181 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
      241 taactacgat acgggagggc ttaccatctg gcccggcagt accggcataa ccaagcctat
      301 gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat
      361 acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc
      421 gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcttg cgggatatcc
      481 gtctagatag tcatcgctca tccctcacct caaccgtttt gctgcctaac gaggtagtct
      541 aagaccgcgc caattctgtc gagtgccgcg ctagtggtac gttggtactc atcaagccgt
      601 gcttcggttt gtaattgcct agcggtaaga atttcaaagt tctgctgatg gatttgaact
      661 gtttgcaaca atgcttctgc ggttgcgcgg agttctgtaa ccgatgtgcg gaggtcggca
      721 ttcactgcaa cttggctgtc taacgcagct tctattctgg ctagtctgtc ttcgctcatg
      781 ctgc
//
