LOCUS       sequence-131920-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flag tag with 3 residue spacer fused to the N-terminus of Lamp2b, a 
            transmembrane exosomal protein. 3 residue spacer then HA tag fused 
            to the C-terminus of Lamp2b..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Hung ME, Leonard JN
  TITLE     Stabilization of exosome-targeting peptides via engineered 
            glycosylation.
  JOURNAL   J Biol Chem. 2015 Mar 27;290(13):8166-72. doi: 
            10.1074/jbc.M114.621383. Epub 2015 Feb 5.
  PUBMED    25657008
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.621383"; journalName: "J Biol Chem"; date: 
            "2015-03-27- 27"; volume: "290"; issue: "13"; pages: "8166-72"
COMMENT     Sequence Label: pcDNA Flag Lamp2b-HA
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      77..86
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             167..190
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agtgccgccg ccatggtgtg cttccgcctc ttcccggttc cgggctcagg
      121 gctcgttctg gtctgcctag tcctgggagc tgtgcggtct tatgcagact ataaagatga
      181 cgatgacaag ggctcgagtt tggaacttaa tttgacagat tcagaaaatg ccacttgcct
      241 ttatgcaaaa tggcagatga atttcacagt tcgctatgaa actacaaata aaacttataa
      301 aactgtaacc atttcagacc atggcactgt gacatataat ggaagcattt gtggggatga
      361 tcagaatggt cccaaaatag cagtgcagtt cggacctggc ttttcctgga ttgcgaattt
      421 taccaaggca gcatctactt attcaattga cagcgtctca ttttcctaca acactggtga
      481 taacacaaca tttcctgatg ctgaagataa aggaattctt actgttgatg aacttttggc
      541 catcagaatt ccattgaatg acctttttag atgcaatagt ttatcaactt tggaaaagaa
      601 tgatgttgtc caacactact gggatgttct tgtacaagct tttgtccaaa atggcacagt
      661 gagcacaaat gagttcctgt gtgataaaga caaaacttca acagtggcac ccaccataca
      721 caccactgtg ccatctccta ctacaacacc tactccaaag gaaaaaccag aagctggaac
      781 ctattcagtt aataatggca atgatacttg cctgctggct accatggggc tgcagctgaa
      841 catcactcag gataaggttg cttcagttat taacatcaac cccaatacaa ctcactccac
      901 aggcagctgc cgttctcaca ctgctctact tagactcaat agcagcacta ttaagtatct
      961 agactt
//
