LOCUS       sequence-131924-         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag 
            tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 
            residue spacer, HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Hung ME, Leonard JN
  TITLE     Stabilization of exosome-targeting peptides via engineered 
            glycosylation.
  JOURNAL   J Biol Chem. 2015 Mar 27;290(13):8166-72. doi: 
            10.1074/jbc.M114.621383. Epub 2015 Feb 5.
  PUBMED    25657008
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.621383"; journalName: "J Biol Chem"; date: 
            "2015-03-27- 27"; volume: "290"; issue: "13"; pages: "8166-72"
COMMENT     Sequence Label: pcDNA GNSTM-3-Flag-10-Lamp2b-HA
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      77..86
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             191..214
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agtgccgccg ccatggtgtg cttccgcctc ttcccggttc cgggctcagg
      121 gctcgttctg gtctgcctag tcctgggagc tgtgcggtct tatgcaggta actcgactat
      181 gggcagtgga gactataaag atgacgatga caagggcagt ggatctggat ccggtggctc
      241 gagtttggaa cttaatttga cagattcaga aaatgccact tgcctttatg caaaatggca
      301 gatgaatttc acagttcgct atgaaactac aaataaaact tataaaactg taaccatttc
      361 agaccatggc actgtgacat ataatggaag catttgtggg gatgatcaga atggtcccaa
      421 aatagcagtg cagttcggac ctggcttttc ctggattgcg aattttacca aggcagcatc
      481 tacttattca attgacagcg tctcattttc ctacaacact ggtgataaca caacatttcc
      541 tgatgctgaa gataaaggaa ttcttactgt tgatgaactt ttggccatca gaattccatt
      601 gaatgacctt tttagatgca atagtttatc aactttggaa aagaatgatg ttgtccaaca
      661 ctactgggat gttcttgtac aagcttttgt ccaaaatggc acagtgagca caaatgagtt
      721 cctgtgtgat aaagacaaaa cttcaacagt ggcacccacc atacacacca ctgtgccatc
      781 tcctactaca acacctactc caaaggaaaa accagaagct ggaacctatt cagttaataa
      841 tggcaatgat acttgcctgc tggctaccat ggggctgcag ctgaacatca ctcaggataa
      901 ggttgcttca gttattaaca tcaaccccaa tacaactcac tccacaggca gctgccgttc
      961 tcacactgct ctactta
//
