LOCUS       sequence-131930-        1036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression vector with 1D4 epitope added as a C-terminal tag, 
            allowing for in vitro expression of any visual pigment or GPCR of 
            interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Morrow JM, Chang BS
  TITLE     The p1D4-hrGFP II expression vector: a tool for expressing and 
            purifying visual pigments and other G protein-coupled receptors.
  JOURNAL   Plasmid. 2010 Nov;64(3):162-9. doi: 10.1016/j.plasmid.2010.07.002. 
            Epub 2010 Jul 11.
  PUBMED    20627111
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.plasmid.2010.07.002"; journalName: "Plasmid"; date: 
            "2010-11"; volume: "64"; issue: "3"; pages: "162-9"
COMMENT     Sequence Label: p1D4-hrGFP II
FEATURES             Location/Qualifiers
     source          1..1036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             182..253
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     misc_feature    289..861
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(456..473)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     683..702
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttctggaggc ccgggctttc agggtaccga
      121 agaaggatcc aaggaggaat tcgacagaga ccagccaagt ggcgcctgcc taagcggccg
      181 cgactacaag gatgacgatg acaaggatta caaagacgac gatgataagg actataagga
      241 tgatgacgac aaataatagc aattcctcga cgactgcata gggttacccc cctctccctc
      301 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
      361 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
      421 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
      481 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
      541 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
      601 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
      661 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
      721 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
      781 atgtgtttag tcgaggttaa aaaacgtcta ggccccccga accacgggga cgtggttttc
      841 ctttgaaaaa cacgatgata atgaccatgg tgagcaagca gatcctgaag aacaccggcc
      901 tgcaggagat catgagcttc aaggtgaacc tggagggcgt ggtgaacaac cacgtgttca
      961 ccatggaggg ctgcggcaag ggcaacatcc tgttcggcaa ccagctggtg cagatccgcg
     1021 tgaccaaggg cgcccc
//
