LOCUS       sequence-131931-        1084 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression vector with 1D4 epitope added as a C-terminal tag, 
            allowing for in vitro expression of any visual pigment or GPCR of 
            interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Morrow JM, Chang BS
  TITLE     The p1D4-hrGFP II expression vector: a tool for expressing and 
            purifying visual pigments and other G protein-coupled receptors.
  JOURNAL   Plasmid. 2010 Nov;64(3):162-9. doi: 10.1016/j.plasmid.2010.07.002. 
            Epub 2010 Jul 11.
  PUBMED    20627111
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.plasmid.2010.07.002"; journalName: "Plasmid"; date: 
            "2010-11"; volume: "64"; issue: "3"; pages: "162-9"
COMMENT     Sequence Label: p1D4-hrGFP II
FEATURES             Location/Qualifiers
     source          1..1084
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             145..864
                     /codon_start=1
                     /product="humanized Renilla green fluorescent protein"
                     /label=hrGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFGN
                     QLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLRYE
                     DGGLVEIRSDINLIEGMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGVLVG
                     QVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQHETAI
                     AQLTSLGKPLGSLHEWV"
     primer_bind     complement(895..915)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(895..913)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(933..950)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    939..1050
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
       61 gtgtttagtc gaggttaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
      121 ttgaaaaaca cgatgataat gaccatggtg agcaagcaga tcctgaagaa caccggcctg
      181 caggagatca tgagcttcaa ggtgaacctg gagggcgtgg tgaacaacca cgtgttcacc
      241 atggagggct gcggcaaggg caacatcctg ttcggcaacc agctggtgca gatccgcgtg
      301 accaagggcg cccccctgcc cttcgccttc gacatcctga gccccgcctt ccagtacggc
      361 aaccgcacct tcaccaagta ccccgaggac atcagcgact tcttcatcca gagcttcccc
      421 gccggcttcg tgtacgagcg caccctgcgc tacgaggacg gcggcctggt ggagatccgc
      481 agcgacatca acctgatcga ggggatgttc gtgtaccgcg tggagtacaa gggccgcaac
      541 ttccccaacg acggccccgt gatgaagaag accatcaccg gcctgcagcc cagcttcgag
      601 gtggtgtaca tgaacgacgg cgtgctggtg ggccaggtga tcctggtgta ccgcctgaac
      661 agcggcaagt tctacagctg ccacatgcgc accctgatga agagcaaggg cgtggtgaag
      721 gacttccccg agtaccactt catccagcac cgcctggaga agacctacgt ggaggacggc
      781 ggcttcgtag agcagcacga gaccgccatc gcccagctga ccagcctggg caagcccctg
      841 ggcagcctgc acgagtgggt gtaagctcga gcatgcatct agagggccct attcccttta
      901 gtgagggtta attgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc
      961 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctctcactgt
     1021 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     1081 gggg
//
