LOCUS       sequence-131953-        1233 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Maltose Binding Protein linked to SnoopTag, 
            a peptide tag forming a spontaneous covalent bond with its protein 
            partner Snoopcatcher..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Veggiani G, Nakamura T, Brenner MD, Gayet RV, Yan J, Robinson CV, 
            Howarth M
  TITLE     Programmable polyproteams built using twin peptide superglues.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214.
  PUBMED    26787909
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2016 Jan 19. pii: 201519214."
COMMENT     Sequence Label: pET28a SnoopTag-MBP
FEATURES             Location/Qualifiers
     source          1..1233
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             67..102
                     /label=SnoopTag
     CDS             121..1218
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="KIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEE
                     KFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLI
                     AYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAA
                     DGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGET
                     AMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLE
                     NYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFW
                     YAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     1192..1215
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atgggaaaac tgggcgatat tgaatttatt aaagtgaaca aaggtagtgg tgaaagtggt
      121 aaaatcgaag aaggtaaact ggtaatctgg attaacggcg ataaaggcta taacggtctc
      181 gctgaagtcg gtaagaaatt cgagaaagat accggaatta aagtcaccgt tgagcatccg
      241 gataaactgg aagagaaatt cccacaggtt gcggcaactg gcgatggccc tgacattatc
      301 ttctgggcac acgaccgctt tggtggctac gctcaatctg gcctgttggc tgaaatcacc
      361 ccggacaaag cgttccagga caagctgtat ccgtttacct gggatgccgt acgttacaac
      421 ggcaagctga ttgcttaccc gatcgctgtt gaagcgttat cgctgattta taacaaagat
      481 ctgctgccga acccgccaaa aacctgggaa gagatcccgg cgctggataa agaactgaaa
      541 gcgaaaggta agagcgcgct gatgttcaac ctgcaagaac cgtacttcac ctggccgctg
      601 attgctgctg acgggggtta tgcgttcaag tatgaaaacg gcaagtacga cattaaagac
      661 gtgggcgtgg ataacgctgg cgcgaaagcg ggtctgacct tcctggttga cctgattaaa
      721 aacaaacaca tgaatgcaga caccgattac tccatcgcag aagctgcctt taataaaggc
      781 gaaacagcga tgaccatcaa cggcccgtgg gcatggtcca acatcgacac cagcaaagtg
      841 aattatggtg taacggtact gccgaccttc aagggtcaac catccaaacc gttcgttggc
      901 gtgctgagcg caggtattaa cgccgccagt ccgaacaaag agctggcaaa agagttcctc
      961 gaaaactatc tgctgactga tgaaggtctg gaagcggtta ataaagacaa accgctgggt
     1021 gccgtagcgc tgaagtctta cgaggaagag ttggcgaaag atccacgtat tgccgccact
     1081 atggaaaacg cccagaaagg tgaaatcatg ccgaacatcc cgcagatgtc cgctttctgg
     1141 tatgccgtgc gtactgcggt gatcaacgcc gccagcggtc gtcagactgt cgatgaagcc
     1201 ctgaaagacg cgcagactaa ttcgagctcg taa
//
