LOCUS       sequence-131957-         474 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of SpyCatcher with a peptide tag enabling 
            site-specific biotinylation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Veggiani G, Nakamura T, Brenner MD, Gayet RV, Yan J, Robinson CV, 
            Howarth M
  TITLE     Programmable polyproteams built using twin peptide superglues.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214.
  PUBMED    26787909
REFERENCE   2  (bases 1 to 474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2016 Jan 19. pii: 201519214."
COMMENT     Sequence Label: AviTag-SpyCatcher
FEATURES             Location/Qualifiers
     source          1..474
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             37..81
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             106..126
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             133..471
                     /codon_start=1
                     /product="truncated version of the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyCatcher
                     /note="forms a covalent isopeptide bond with the SpyTag 
                     peptide (Zakeri et al., 2012)"
                     /translation="VDTLSGLSSEQGQSGDMTIEEDSATHIKFSKRDEDGKELAGATME
                     LRDSSGKTISTWISDGQVKDFYLYPGKYTFVETAAPDGYEVATAITFTVNEQGQVTVNG
                     KATKGDAHI"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac ggcagcggcc tgaacgatat ttttgaagcg
       61 cagaaaattg aatggcatga aggcgattac gacatcccaa cgaccgaaaa cctgtatttt
      121 cagggcgcca tggttgatac cttatcaggt ttatcaagtg agcaaggtca gtccggtgat
      181 atgacaattg aagaagatag tgctacccat attaaattct caaaacgtga tgaggacggc
      241 aaagagttag ctggtgcaac tatggagttg cgtgattcat ctggtaaaac tattagtaca
      301 tggatttcag atggacaagt gaaagatttc tacctgtatc caggaaaata tacatttgtc
      361 gaaaccgcag caccagacgg ttatgaggta gcaactgcta ttacctttac agttaatgag
      421 caaggtcagg ttactgtaaa tggcaaagca actaaaggtg acgctcatat ttaa
//
