LOCUS       sequence-131962-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This pUC19 vector contains a theophylline binding riboswitch, 
            controlling the translation initiation rate of luciferase gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Espah Borujeni A, Mishler DM, Wang J, Huso W, Salis HM
  TITLE     Automated physics-based design of synthetic riboswitches from 
            diverse RNA aptamers.
  JOURNAL   Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
  PUBMED    26621913
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Nov 30. pii: gkv1289."
COMMENT     Sequence Label: AEB-Theo27 pUC19
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(9..14,15..30,31..37)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     primer_bind     complement(184..202)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ccgagctgtt gacaattaat catcggctcg tataatgtgt gggacgctac tcatctttta
       61 ggtgatacca gcatcgtctt gatgcccttg gcagcaccag ctgctaggcc gttcaagatg
      121 gaagacgcca aaaacataaa gaaaggcccg gcgccattct atcctctaga ggatggaacc
      181 gctggagagc aactgcataa ggctatgaag agatacgccc tggttcctgg aacaattgct
      241 tttacagatg cacatatcga ggtgaacatc acgtacgcgg aatacttcga aatgtccgtt
      301 cggttggcag aagctatgaa acgatatggg ctgaatacaa atcacagaat cgtcgtatgc
      361 agtgaaaact ctcttcaatt ctttatgccg gtgttgggcg cgttatttat cggagttgca
      421 gttgcgcccg cgaacgacat ttataatgaa cgtgaattgc tcaacagtat gaacatttcg
      481 cagcctaccg tagtgtttgt ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa
      541 aaattaccaa taatccagaa aattattatc atggattcta aaacggatta ccagggattt
      601 cagtcgatgt acacgttcgt cacatctcat ctacctcccg gttttaatga atacgatttt
      661 gtaccagagt cctttgatcg tgacaaaaca attgcactga taatgaattc ctctggatct
      721 actgggttac ctaagggtgt ggcccttccg catagaactg cctgcgtcag attctcgcat
      781 gccagagatc ctatttttgg caatcaaatc attccggata ctgcgatttt aagtgttgtt
      841 ccattccatc acggttttgg aatgtttact acactcggat atttgat
//
