LOCUS       sequence-13197-e        4713 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 4713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1510-HQ858841
FEATURES             Location/Qualifiers
     source          1..4713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2270..2369)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2386..2405)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2387..2405)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2410..2426)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2410..2426)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2528..2547)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2539..3348
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2606..2625)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3495..4083
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3984..4003
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4237..4254
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4413..4440)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4532..4618)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4667..4689
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4681..4698
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4682..4698
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atggaggcgg acggtgccgc ggctgcggcg gcggcggggg aggcgagcac ggaggccggc
      181 gcgaggcccc tggcgccaga ggaggaggcg ctgcggcgca acaccgactg cgtctacttc
      241 ctcgcctcgc ccctcacatg caagaagggg aacgaatgtg attttcgtca cagtgataat
      301 gctcgtatga accctagaga ctgctggtac tggttaaata gcaattgctt gaatccaaaa
      361 tgtccgtttc gccatccgcc aattgatggt atgtttggtg ctccaactac tggaatgcct
      421 gcagtttctt cacactacgc tccatttaac tctggaaagc agttggttcc atgctattac
      481 tttaaaaagg gaaactgctt gaaaggtgac agatgcgcat tttatcatgg accacaatca
      541 gtgggaaata atccatcaga acaggtggta aaagtttctt ccttaccttt ggagcagctt
      601 cagactcaaa agaatgattt gttgggcatt aaagattctg tgcagtctac taattccatt
      661 cagcatggtg ctccaataac taatgagagg ggcaaaacag ccgttgatcg gtcaacggtt
      721 aattctgcca ggacagctac agttgcaata ccggttgctt ccaatgcaat gtcttgtcct
      781 aagtcagaga aggtaaaaag tagtacgcca gcagcactta aggaaagctt cactacttct
      841 tcaggtgggg atcatcctga gtgctaccag aaccattttc caatggatag tgatcctgtg
      901 cgagattgga accaaagtta tgaaatgccc cctgcagatg atctgcccca gaacagtagg
      961 gaggctgatg aacttttggg tgaatcttca ccaggttttg atgttctcgt ggacaatgat
     1021 gcagatggtg ctgcttattt acatgatgaa gattttggag gagatatgta tccagtggaa
     1081 gattatgaat atgctccagc tgattttgat gttcgtgcac atcatgaaag agagcggttc
     1141 aatggtatgg atgagcaaga ccagatggga catatgtatg atggctacga gagaaaacgg
     1201 cgtagatcat ctgagaggag catggaacgg cctttccact cagaaagaag gtttctgcaa
     1261 agagatagag accgtgtcga gatggatgga tcggatttac gtcatcggct ccgtaggaga
     1321 agaataaatg agtcttcatt ggcaattagt ccagaacgca atggagagca acggcggagg
     1381 gatgagcgct acagggaaag ggcacatggt caccgttcac acagggatca tcaccagagc
     1441 tctcgaggaa gcactcttag ctctcgtctg caaggcagaa taaagcttcc tggaagatct
     1501 cctgacagag ttgatactcg ctccgagaaa gagcgagata gaagaagact ccgggacagg
     1561 ctgtctccag ttaggcgcac ggaatttcag ggtaccaggc atagggaggc aggacaacat
     1621 gaagagcaga cccagcggag atcaagtgaa cttgctttag gttccaggaa tgcagatggc
     1681 cagcatttga caaaggatgt gcctgactca cacaacttcc ctcatcgcaa aaactcgagg
     1741 gactccagta aggccaatgg aagtgttgag cctgaagcat cgcttgattt tgagggccca
     1801 aagcccctta gtgtcatctt acagcggaaa agagaggcag catgggccaa tggcacatct
     1861 gcctgtagcc ccaaacaaga taaatctgcc gaggtctcac acaggcaagc ttctctggct
     1921 gaagctgaga aggaaggtga caacataatt agttctgatg aatacaaaag tggatctggc
     1981 gatgaagaat tcagagacga aggccacatc cctgtggagg gccatgggca atcttcatct
     2041 catggtgaca aacttgaggc tgaagatatt attgaggtgg atcccgtgga aaatcaagat
     2101 gctgataatt atgaccagag ggagggggag tcctactatg agcctatcga aggccaggac
     2161 tacaaatccg atgacgagga tgcttatgaa gatgatgatg aagagtatga tgacgatgat
     2221 gattttgctc ggaaagtagg ggttgttttc tctaagggtg ggcgcgccga cccagctttc
     2281 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
     2341 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg
     2401 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
     2461 tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac
     2521 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat
     2581 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     2641 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     2701 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     2761 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     2821 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca
     2881 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     2941 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     3001 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     3061 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     3121 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     3181 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     3241 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     3301 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     3361 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga
     3421 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag
     3481 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     3541 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3601 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     3661 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3721 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3781 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3841 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc
     3901 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3961 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     4021 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     4081 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     4141 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     4201 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     4261 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     4321 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt
     4381 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct
     4441 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt
     4501 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg
     4561 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat
     4621 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt
     4681 tgtaaaacga cggccagtct taagctcggg ccc
//
