LOCUS       sequence-131975-         636 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This E. coli DH10B strain harbors a shuttle vector plasmid that 
            expresses the Mixed Feedback Loop version of the UBER system with a 
            T7RNAP translation rate of 300 and a TetR translation rate of 
            35149..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Kushwaha M, Salis HM
  TITLE     A portable expression resource for engineering cross-species genetic
            circuits and pathways.
  JOURNAL   Nat Commun. 2015 Jul 17;6:7832. doi: 10.1038/ncomms8832.
  PUBMED    26184393
REFERENCE   2  (bases 1 to 636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-07-17"; volume: "6:7832. doi"; pages: " 
            10.1038/ncomms8832"
COMMENT     Sequence Label: MK1274
FEATURES             Location/Qualifiers
     source          1..636
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     21..40
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        21..39
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             128..148
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tatcattgat aggtataaat taatacgact cactataggg agacctatca gtgatagagg
       61 atccaaccga gcccaatata ggactcaggg tgccaaaaaa atgtctacca ccatgggaat
      121 tcaacctcct aaaaagaaac gtaaagttaa tactattaat attgctaaaa atgacttctc
      181 agatattgaa ttagcagcca ttccatttaa tacattagca gatcactatg gtgaacgttt
      241 agcacgtgaa cagttagcat tagaacatga atcatatgaa atgggtgaag cacgttttcg
      301 taagatgttc gagcgtcagt taaaagcagg tgaagttgca gataatgcag cagccaaacc
      361 tttaattact acattattac ctaaaatgat tgctcgtatt aacgattggt ttgaagaggt
      421 taaagcaaag cgtggtaaac gtcctacagc atttcagttc ttacaagaaa tcaaacctga
      481 agcagttgca tatattacta ttaaaacaac attagcatgt ttaacatcag cagataatac
      541 aacagttcaa gcagttgcat cagcaattgg tcgtgcaatt gaagatgaag cacgttttgg
      601 tcgtattcgt gatttagaag ccaaacattt taaaaa
//
