LOCUS       sequence-13198-e        4269 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1530-HQ858842
FEATURES             Location/Qualifiers
     source          1..4269
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1826..1925)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1942..1961)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1943..1961)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1966..1982)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1966..1982)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2084..2103)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2095..2904
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2162..2181)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3051..3639
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3540..3559
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3793..3810
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3969..3996)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4088..4174)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4223..4245
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4237..4254
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4238..4254
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atgggctccc agaaccgccc cccaccaccg cgcaagaggc agccgccgcc gccggaggac
      181 cacctcgtca cctacaagcg ccgccgctcc aaagaaacac agcctttgcc actcatggcc
      241 aacggcgcca attctaagaa agacgccaag gcccaacatt ggattagctg gagggacact
      301 ctccacggct tcctccaatc ccctgctatt agccagggtg gaggaattca gacctgcatc
      361 cgtcatgctc tccaacacaa tccttgcttg ctcaccaatg gtgttgttgt tcacactgaa
      421 ttcaaaggta acccagctca ttcccaagga gaggaagcaa aagtgcagca ccctaatggt
      481 gctgctggag gcaaggtggt ttctgcagat gctgctatac aagatgcggc tgccgcagct
      541 tcttccgaag ctaacaaggc aatgtgtaat aatgccctct tcgtcatttt ggtctcccag
      601 aaatttgcct tgttgtgcca tttgctactt gggaccttcc atgtcaataa acctggtgat
      661 gtcatcgact tggaaaaaat cgacgccaag atgagaaatg gagactatgc ccacaaccct
      721 gcactatttg acgatgatat ccagcagatg tgggagaagt ttgagcaagt tggccaagag
      781 atgacaggtc tagcgagcaa cctttcaacc atttcacgag tttcatacca aaagcaggct
      841 tctggatttt ctgaagctga ggtggctgag cacagaatag aggaaataag tttgccgggt
      901 gctgtccacg ttgtcacaaa ggagtcgact accaccgtgc agttggcccc atgtgattct
      961 agtcattcta caataccgaa acgaactgtg ccacctggac gtgatctgtg cccttgcgat
     1021 ggttgtggca ccaaggtaga tgttgaagaa ggcctaatct gtgatgaatg tgacaccatg
     1081 taccactttg catgtgtcaa gctactcaat cctgatatta agcaagtccc agcaatctgg
     1141 cattgttcaa cctgcagctt caagaaaaaa gaattggctg cagataccac gaataatgtt
     1201 gcccatgact gcttgcatgg tggtaactgt gttttgtgtg accagctcga gctggtgaag
     1261 acagaagaag aagatcccaa gcttcccata aaaattgaat tagctgaaga aagagagggg
     1321 agctccgtct caagcatggg ggaagacaat gaaccagacc tgtcaacaac tgccctgtca
     1381 aacttgtgca aacactgtgg cacatgcgaa gacgatgaca agagattcat ggtatgcgga
     1441 catccttact gcgtttacaa gttctatcat atccgatgcc tgaaaacaag ccagcttgca
     1501 attgagcaac aaaagaagct tggttgctgg tactgcccct cttgcctctg cagagggtgc
     1561 ttccaagaca aggatgatga ccagatagtc atgtgtgatg gctgtgatga aggttatcac
     1621 atatattgca tgagaccagc acgcaacact atccccaaag gtaagtggta ctgtacattt
     1681 tgcaagatcc gcagggcagc ggaaggaatg cataagtatg aggattctgt gctgaaaata
     1741 catgggaata gtaagcatgc ttgtaatgtg aatcagtcga aggattctga aggtgatggt
     1801 actgaaaaga agggtgggcg cgccgaccca gctttcttgt acaaagttgg cattataaga
     1861 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
     1921 atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
     1981 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     2041 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     2101 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat
     2161 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat
     2221 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     2281 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     2341 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa
     2401 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     2461 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     2521 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     2581 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa
     2641 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     2701 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     2761 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     2821 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     2881 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc
     2941 attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac
     3001 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3061 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3121 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3181 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     3241 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3301 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3361 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3421 ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3481 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3541 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3601 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3661 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     3721 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     3781 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     3841 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     3901 aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta
     3961 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt
     4021 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc
     4081 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
     4141 cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg
     4201 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag
     4261 ctcgggccc
//
