LOCUS       sequence-131981-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The pAK plasmid expresses GFP driven by a T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Kushwaha M, Salis HM
  TITLE     A portable expression resource for engineering cross-species genetic
            circuits and pathways.
  JOURNAL   Nat Commun. 2015 Jul 17;6:7832. doi: 10.1038/ncomms8832.
  PUBMED    26184393
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-07-17"; volume: "6:7832. doi"; pages: " 
            10.1038/ncomms8832"
COMMENT     Sequence Label: MK969
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      724..771
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aagggtgaag aattatttac aggtgttgtt cctattttag ttgaattaga tggtgatgtt
       61 aatggtcata agttctcagt ttcaggtgaa ggtgaaggtg atgcaacata tggtaagtta
      121 acattaaagt tcatttgtac aacaggtaaa ttacctgttc cttggcctac attagttaca
      181 acattaacat atggtgttca atgtttttca cgttatcctg atcatatgaa acaacatgac
      241 ttctttaaat cagcaatgcc agaaggttac gttcaagaac gtaccatctt ttttaaagat
      301 gatggtaact ataaaacacg tgcagaagtt aagttcgaag gtgatacatt agttaatcgt
      361 attgaattaa aaggtattga cttcaaagaa gatggtaata ttttaggtca taaattagaa
      421 tataactata attcacataa tgtttatatt atggcagata aacagaaaaa tggtattaaa
      481 gttaacttca agatacgtca taatattgaa gatggttcag ttcagttagc agatcattat
      541 caacagaata ctcctattgg tgatggtcct gttttattac ctgataatca ttacttatca
      601 acccaatcag cattatcaaa agatcctaat gaaaaacgtg atcatatggt tttattagag
      661 ttcgttacag cagcaggtat tacattaggt atggatgaat tatataaatg ataatctaga
      721 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgcctagc
      781 gatgaatggc gaatggaaat tgtaagcgtt a
//
